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产品描述：人富亮氨酸重复序列蛋白17(LRRC17)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的LRRC17含量。试剂盒检测范围为23.44 pg/mL-1500 pg/mL，该产品适用于基础科研领域，可用于探究LRRC17在肿瘤微环境、免疫调节或神经退行性疾病中的表达变化，为相关分子机制研究提供定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：LRRC17 ELISA Kit; P37NB ELISA Kit; UNQ3076/PRO9909 ELISA Kit; Leucine-rich repeat-containing protein 17 ELISA Kit; p37NB ELISA Kit
缩写：LRRC17
Uniprot No.：Q8N6Y2
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.44 pg/mL-1500 pg/mL
灵敏度：5.86 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human LRRC17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-101 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 85-98 | |||||
| 1:4 | Average % | 86 | ||||
| Range % | 80-92 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of human LRRC17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-99 | ||||
| EDTA plasma (n=4) | 95 | 91-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15001.7111.6721.6921.5627501.4261.3961.4111.2813751.0121.0041.0080.878187.50.7750.7560.7660.63693.750.5830.5440.5640.43446.870.3740.3590.3670.23723.440.2620.2470.2550.12500.1330.1270.130 | ||||||
货期：3-5 working days
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