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产品描述：人低密度脂蛋白受体相关蛋白6(LRP-6)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、组织匀浆、细胞裂解物样本中的LRP6含量。LRP6，全称低密度脂蛋白受体相关蛋白6，是Wnt/β-catenin信号通路的重要协同受体，参与调节胚胎发育和代谢。其基因多态性与代谢综合征、糖尿病、高血脂症和动脉粥样硬化等疾病密切相关，研究其机制有助于疾病诊断和治疗。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于基础科研中探索LRP - 6蛋白在疾病模型中的表达调控，例如分析癌症细胞系中Wnt通路活性、评估代谢综合征动物模型的受体表达水平变化，或研究干细胞分化过程中LRP - 6的动态变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ADCAD2 ELISA Kit; C030016K15Rik ELISA Kit; Cd ELISA Kit; FLJ90062 ELISA Kit; FLJ90421 ELISA Kit; LDL receptor related protein 6 ELISA Kit; Low density lipoprotein receptor related protein 6 ELISA Kit; Low-density lipoprotein receptor-related protein 6 ELISA Kit; LRP-6 ELISA Kit; LRP6 ELISA Kit; LRP6_HUMAN ELISA Kit; OTTHUMP00000238979 ELISA Kit; OTTHUMP00000238980 ELISA Kit; OTTHUMP00000238982 ELISA Kit; STHAG7 ELISA Kit
缩写：LRP6
Uniprot No.：O75581
种属：Homo sapiens (Human)
样本类型：serum, tissue homogenates, cell lysates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of Human LRP-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 83-91 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 86-97 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 88-98 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 98-104 | |||||
回收率：
| The recovery of Human LRP-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 88-98 | ||||
| EDTA plasma (n=4) | 86 | 82-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.6252.7292.6772.494102.1462.0472.0971.91451.4441.4631.4541.2712.50.9120.9280.9200.7371.250.5970.5860.5920.4090.6250.4010.4160.4090.2260.3120.2870.2750.2810.09800.1820.1830.183 | ||||||
货期：3-5 working days
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