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产品描述：人脂多糖/内毒素(LPS)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LPS含量。LPS即脂多糖，是革兰氏阴性菌细胞壁成分。它可激活机体免疫反应，启动炎症信号通路，在免疫调节、疾病发生发展中扮演重要角色。研究其作用机制有助于理解炎症性疾病发病原理，为开发针对性治疗方案提供依据。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于研究感染性疾病模型中内毒素释放规律、炎症信号通路激活机制，或用于药物开发过程中化合物对LPS介导炎症反应的调控作用评估，为免疫学、微生物学及药理学等领域的基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：LPS
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human LPS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 82-94 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 89-99 | |||||
回收率：
| The recovery of human LPS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 90-97 | ||||
| EDTA plasma (n=4) | 88 | 82-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 4002.8092.9602.8852.720 2002.2272.3662.2972.132 1001.5151.5561.5361.371 500.7890.7550.7720.607 250.4320.4700.4510.286 12.50.3030.3250.3140.149 6.250.2350.2320.2340.069 00.1640.1650.165 | ||||||
本试剂盒所含材料：
An assay plate --- The 96-well plate is composed of 12 x 8 strip plates and can be taken apart.
Two bottles of lyophilized standard --- Reconstitute one bottle of the standard with sample diluent at dilution series and draw the standard curve.
Biotin-labeled LPS antibody (100 x concentrate) 1 x 120 μl --- Act as the detection antibody and need to be diluted before use.
HRP-avidin (100 x concentrate) 1 x 120 μl --- Catalyze the TMB substrate for color development and need to be diluted before use.
Biotin-antibody Diluent 1 x 15 ml --- Dilute the Biotin-antibody.
HRP-avidin Diluent 1 x 15 ml --- Dilute the HRP-avidin.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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