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产品描述：小鼠赖氨酰氧化酶同源物2(LOXL2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LOXL2含量。LOXL2是一种赖氨酰氧化酶样2蛋白。其背景在于它在许多生理和病理过程中发挥作用，如组织重塑。研究机制方面，它参与细胞外基质交联等过程，在肿瘤发生、发展、转移等环节有影响，是肿瘤等疾病潜在治疗靶点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于基础科研中对LOXL2表达水平的定量分析，例如探究纤维化疾病模型中的分子机制、肿瘤微环境调控研究或组织修复相关信号通路探索，为体外实验提供可靠的蛋白浓度数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Loxl2Lysyl oxidase homolog 2 ELISA Kit; EC 1.4.3.13 ELISA Kit; Lysyl oxidase-like protein 2 ELISA Kit
缩写：LOXL2
Uniprot No.：P58022
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse LOXL2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 82-88 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 91-97 | |||||
| 1:4 | Average % | 103 | ||||
| Range % | 97-109 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 92-106 | |||||
回收率：
| The recovery of mouse LOXL2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-102 | ||||
| EDTA plasma (n=4) | 89 | 83-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.3952.6042.5002.3315001.5291.5791.5541.3852500.9600.9690.9650.7961250.6230.5880.6060.43762.50.3310.3500.3410.17231.250.2650.2690.2670.09815.60.2070.2200.2140.04500.1620.1750.169 | ||||||
货期：3-5 working days
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