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产品描述：人赖氨酰氧化酶同源物1(LOXL1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的LOXL1含量。LOXL1是重要靶点。它参与细胞外基质交联等过程。在一些疾病如剥脱性青光眼、癌症等研究中受关注。研究机制聚焦其在疾病发生发展里的功能，如影响细胞迁移、增殖，探索以它为靶点开发诊断标志物和治疗药物。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于科研领域探索LOXL1在纤维化疾病模型中的表达调控、肿瘤微环境基质硬化机制研究，或作为评估抗纤维化药物效果的分子检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：LOXL1 ELISA Kit; LOXLLysyl oxidase homolog 1 ELISA Kit; EC 1.4.3.- ELISA Kit; Lysyl oxidase-like protein 1 ELISA Kit; LOL ELISA Kit
缩写：LOXL1
Uniprot No.：Q08397
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human LOXL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 86-94 | |||||
| 1:2 | Average % | 88 | ||||
| Range % | 83-92 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 92-102 | |||||
回收率：
| The recovery of human LOXL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-98 | ||||
| EDTA plasma (n=4) | 86 | 82-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.4382.5462.4922.36451.5281.4251.4771.3492.50.8560.9020.8790.7511.250.5230.5470.5350.4070.6250.3180.3330.3260.1980.3120.2550.2640.2600.1320.1560.2040.2080.2060.07800.1270.1280.128 | ||||||
货期：3-5 working days
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