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产品描述：人核纤层蛋白A/C(LMNA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的LMNA含量。LMNA基因编码核纤层蛋白A/C，其突变会导致核纤层结构和功能异常，进而引发多种疾病。研究中发现它与衰老、肌肉疾病等密切相关，其机制主要围绕维持细胞核结构稳定、调控基因表达、参与信号转导等展开，是当前生物医学研究热点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，可广泛应用于细胞生物学研究中LMNA功能探索、疾病模型构建（如衰老相关表型分析、肌肉或心血管疾病机制研究）以及药物干预实验中靶点蛋白动态监测。为深入解析LMNA在生理病理过程中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
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缩写：LMNA
Uniprot No.：P02545
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human LMNA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 85-91 | |||||
| 1:2 | Average % | 103 | ||||
| Range % | 100-106 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 82-89 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 93-99 | |||||
回收率：
| The recovery of human LMNA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 84 | 81-87 | ||||
| EDTA plasma (n=4) | 94 | 91-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.4342.4682.4512.3025001.6611.6981.6801.5312501.0941.0721.0830.9341250.6560.6270.6420.49362.50.4260.4420.4340.28531.20.3090.3180.3140.16515.60.2580.2480.2530.10400.1500.1480.149 | ||||||
货期：3-5 working days
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