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产品描述：人白细胞免疫球蛋白样受体亚家族B成员4(LILRB4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、细胞裂解物样本中的LILRB4含量。LILRB4是一种重要靶点。它在免疫调节等方面有重要作用，其异常表达与多种疾病尤其是癌症的发生、发展、转移及免疫逃逸相关。研究机制主要聚焦于它与配体相互作用、调控免疫细胞功能及下游信号通路等，为疾病治疗带来新方向。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，本产品适用于免疫学基础研究、肿瘤微环境分析、炎症性疾病机制探索等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD85 antigen-like family member K ELISA Kit; CD85k ELISA Kit; HM18 ELISA Kit; ILT-3 ELISA Kit; ILT3 ELISA Kit; Immunoglobulin-like transcript 3 ELISA Kit; Leukocyte immunoglobulin like receptor subfamily B (with TM and ITIM domains) member 4 ELISA Kit; Leukocyte immunoglobulin-like receptor 5 ELISA Kit; Leukocyte immunoglobulin-like receptor subfamily B member 4 ELISA Kit; LILRB4 ELISA Kit; LILRB5 ELISA Kit; LIR-5 ELISA Kit; LIR5 ELISA Kit; LIRB4_HUMAN ELISA Kit; Monocyte inhibitory receptor HM18 ELISA Kit
缩写：LILRB4
Uniprot No.：Q8NHJ6
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human LILRB4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-98 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 93-102 | |||||
| 1:4 | Average % | 84 | ||||
| Range % | 81-89 | |||||
| 1:8 | Average % | 88 | ||||
| Range % | 83-95 | |||||
回收率：
| The recovery of human LILRB4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 101 | 98-104 | ||||
| EDTA plasma (n=4) | 92 | 89-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.1882.2882.2382.11310001.8331.8991.8661.7415001.3141.3921.3531.2282500.9540.9450.9500.8251250.4780.4600.4690.34462.50.3200.3440.3320.20731.250.2110.2120.2120.08700.1200.1290.125 | ||||||
货期：3-5 working days
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