首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠白血病抑制因子(LIF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LIF含量。LIF即白血病抑制因子，它在胚胎发育、细胞生长分化等多方面发挥重要作用。研究发现，LIF可通过激活相关信号通路，调控细胞的增殖与分化，在维持胚胎干细胞多能性等方面意义重大，在再生医学、肿瘤研究等领域是重要研究靶点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于科研领域中对LIF的定量分析，例如探究炎症反应、干细胞分化机制或评估药物干预对LIF表达的影响；可为体外实验、动物模型研究或细胞培养体系中LIF的动态监测提供可靠工具，满足基础研究中对蛋白定量分析的需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：LifLeukemia inhibitory factor ELISA Kit; LIF ELISA Kit; Differentiation-stimulating factor ELISA Kit; D factor ELISA Kit
缩写：LIF
Uniprot No.：P09056
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse LIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 86-94 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 90-100 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-101 | |||||
回收率：
| The recovery of mouse LIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 84 | 80-88 | ||||
| EDTA plasma (n=4) | 96 | 92-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.7782.6872.7332.5715002.2092.0362.1231.9612501.3771.4211.3991.2371250.6710.7250.6980.53662.50.3430.3170.3300.16831.250.2650.2740.2700.10815.60.2240.2370.2310.06900.1650.1590.162 | ||||||
货期：3-5 working days
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