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产品描述：牛黄体生成素（LH）酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LH含量。LH即黄体生成素，是腺垂体分泌的促性腺激素。其主要作用于性腺，在女性中促进排卵和黄体形成，分泌孕激素；在男性中刺激睾丸间质细胞分泌雄激素。研究多聚焦其分泌调控机制及在生殖内分泌疾病诊治中的应用。试剂盒检测范围为1.25 mIU/mL-100 mIU/mL，广泛应用于动物繁殖生理机制研究、发情周期监测、生殖激素调控实验等科研领域，亦可用于评估饲料添加剂或环境因素对牛群生殖功能的影响，为畜牧养殖优化和基础医学研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：LHBLutropin subunit beta ELISA kit; Luteinizing hormone subunit beta ELISA kit; LH-B ELISA kit; LSH-B ELISA kit; LSH-beta ELISA kit; Lutropin beta chain ELISA kit
缩写：LH
Uniprot No.：P04651
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：1.25 mIU/mL-100 mIU/mL
灵敏度：1.56 mIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine LH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 99 | ||||
| Range % | 95-102 | |||||
| 1:2 | Average % | 87 | ||||
| Range % | 84-90 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 83-94 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 95-105 | |||||
回收率：
| The recovery of bovine LH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 90-95 | ||||
| EDTA plasma (n=4) | 87 | 85-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mIU/mlOD1OD2Average 1000.3610.3680.365 37.50.5380.5670.553 18.750.7810.7650.773 6.251.1561.1261.141 1.251.6621.6981.680 02.1612.1782.170 | ||||||
货期：3-5 working days
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