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产品描述：小鼠富亮氨酸重复LGI家族成员3(LGI3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LGI3含量。小鼠富亮氨酸重复LGI家族成员3（LGI3）是一种神经生物学相关蛋白，参与调节神经元外排作用，主要在脑和肺中表达。其功能可能与抑制性突触组装有关，对神经科学领域的研究具有重要意义。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于神经科学基础研究，可应用于脑组织、外周神经或疾病模型动物血清/血浆中LGI3蛋白表达的定量分析，为探究神经发育异常、癫痫发病机制及药物干预效果提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Lgi3Leucine-rich repeat LGI family member 3 ELISA Kit; Leubrin ELISA Kit; Leucine-rich glioma-inactivated protein 3 ELISA Kit
缩写：LGI3
Uniprot No.：Q8K406
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse LGI3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:2 | Average % | 87 | ||||
| Range % | 83-90 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 98-108 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 85-94 | |||||
回收率：
| The recovery of mouse LGI3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 108 | 104-112 | ||||
| EDTA plasma (n=4) | 100 | 95-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 20002.1062.1382.1221.950 10001.4651.4361.4511.279 5000.8950.9180.9070.735 2500.5510.5360.5440.372 1250.3290.3310.3300.158 62.50.2580.2490.2540.082 31.250.2010.2100.2060.034 00.1700.1730.172 |
货期：3-5 working days
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