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产品描述：人乳糖凝集素-9(LGALS9)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的LGALS9含量。LGALS9，也称为半乳糖凝集素-9，是一种β-半乳糖苷结合蛋白，参与调节细胞间和细胞基质相互作用。它在霍奇金病组织中过度表达，可能与疾病和免疫缺陷的发病机制有关。LGALS9在结直肠癌中表达较低，与免疫细胞浸润呈正相关，可能通过抑制肿瘤细胞的增殖、迁移和侵袭来发挥作用。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品适用于基础科研中探索LGALS9在免疫应答、炎症性疾病或癌症发生发展中的功能机制，例如通过分析疾病模型动物血清/血浆中LGALS9的动态变化，评估组织匀浆中蛋白表达水平，或监测体外培养细胞分泌至上清液的LGALS9含量，为分子机制研究和药物筛选提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：36 kDa beta-galactoside-binding lectin ELISA Kit; Ecalectin ELISA Kit; Gal-9 ELISA Kit; galectin 9 ELISA Kit; Galectin-9 ELISA Kit; galectin9 ELISA Kit; HOM HD 21 ELISA Kit; HOMHD21 ELISA Kit; HUAT ELISA Kit; Lectin galactoside binding soluble 9 ELISA Kit; LEG9_HUMAN ELISA Kit; LGAL S9 ELISA Kit; LGALS 9 ELISA Kit; Lgals9 ELISA Kit; LGALS9A ELISA Kit; MGC117375 ELISA Kit; MGC125973 ELISA Kit; MGC125974 ELISA Kit; Tumor antigen HOM-HD-21 ELISA Kit; UAT ELISA Kit; Urate transporter/channel ELISA Kit; Urate transporter/channel protein ELISA Kit
缩写：LGALS9
Uniprot No.：O00182
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human LGALS9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 86-97 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 95-104 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 83-94 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 87-99 | |||||
回收率：
| The recovery of human LGALS9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 83-93 | ||||
| EDTA plasma (n=4) | 96 | 92-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 20001.9871.9521.9701.814 10001.3451.3761.3611.205 5000.9420.9040.9230.767 2500.5530.5390.5460.390 1250.3550.3720.3640.208 62.50.2610.2770.2690.113 31.250.2020.1890.1960.040 00.1570.1550.156 | ||||||
货期：3-5 working days
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