首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠乳糖凝集素-9(LGALS9)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LGALS9含量。LGALS9，即半乳糖凝集素9，是一种β-半乳糖苷结合蛋白，参与调节细胞-细胞和细胞-基质相互作用。其在霍奇金病组织中过度表达，可能与HRS细胞与其周围细胞的相互作用有关，可能在疾病和/或相关免疫缺陷的发病机制中发挥作用。LGALS9通过与其他分子相互作用，调节T细胞的分化和功能，促进抗病毒和抗肿瘤免疫反应，并抑制炎症反应。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，科研人员可将其应用于免疫机制研究、炎症相关疾病模型分析、药物干预效果评估等基础研究领域，尤其适用于小鼠模型中LGALS9动态变化的纵向监测。为探索LGALS9在肿瘤微环境调控、自身免疫性疾病等领域的功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Lgals9 ELISA Kit; Galectin-9 ELISA Kit; Gal-9 ELISA Kit
缩写：LGALS9
Uniprot No.：O08573
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse LGALS9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 90 | |||||
| Range % | 86-98 | ||||||
| 1:2 | Average % | 99 | |||||
| Range % | 92-107 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 92-98 | ||||||
| 1:8 | Average % | 89 | |||||
| Range % | 82-94 | ||||||
回收率：
| The recovery of mouse LGALS9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 86-93 | |||||
| EDTA plasma (n=4) | 101 | 95-108 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 10002.3392.3142.3272.212 5001.8531.9191.8861.771 2501.3551.3561.3561.241 1250.7420.7240.7330.618 62.50.4230.4110.4170.302 31.20.2890.2850.2870.172 15.60.2220.2330.2280.113 00.1150.1140.115 | |||||||
货期：3-5 working days
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