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Human Leucine-rich glioma-inactivated protein 1(LGI1) ELISA kit

  • 中文名： 人富亮氨酸胶质瘤失活蛋白1(LGI1)酶联免疫试剂盒
货号: IPD-EL012898HU
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人富亮氨酸胶质瘤失活蛋白1(LGI1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LGI1含量。LGI1是重要靶点。它参与神经系统发育和功能维持，如突触形成与稳定。在研究机制上，探究其与神经系统疾病关联，像自身免疫性脑炎。研究其信号通路、抗体结合机制等，有助揭示疾病发病机理并开发新治疗手段。试剂盒检测范围为0.156 ng/mL-10 ng/mL，本产品适用于神经生物学基础研究，可应用于探索LGI1在神经系统疾病模型中的表达调控机制、评估实验动物或细胞模型中LGI1蛋白水平变化等科研场景，为相关疾病的分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    ADLTE ELISA Kit; ADPAEF ELISA Kit; ADPEAF ELISA Kit; Epitempin 1 ELISA Kit; EPITEMPIN ELISA Kit; Epitempin-1 ELISA Kit; EPT ELISA Kit; ETL1 ELISA Kit; IB1099 ELISA Kit; leucine rich glioma inactivated 1 ELISA Kit; Leucine rich glioma-inactivated protein 1 ELISA Kit; Leucine-rich glioma-inactivated protein 1 ELISA Kit; LGI1 ELISA Kit; LGI1_HUMAN ELISA Kit; OTTHUMP00000020121 ELISA Kit; OTTHUMP00000020122 ELISA Kit
  • 缩写：
    LGI1
  • Uniprot No.：
    O95970
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human LGI1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    84


    Range %

    80-90


    1:2

    Average %

    89


    Range %

    84-94


    1:4

    Average %

    95


    Range %

    90-100


    1:8

    Average %

    93


    Range %

    89-98


  • 回收率：

    The recovery of human LGI1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    90-102


    EDTA plasma (n=4)

    88

    83-93


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    10

    3.150

    2.964

    3.057

    2.891


    5

    2.410

    2.317

    2.364

    2.197


    2.5

    1.461

    1.528

    1.494

    1.328


    1.25

    0.896

    0.929

    0.912

    0.746


    0.625

    0.498

    0.511

    0.505

    0.338


    0.312

    0.354

    0.363

    0.359

    0.192


    0.156

    0.243

    0.254

    0.249

    0.082


    0

    0.165

    0.168

    0.167





  • 货期：
    3-5 working days

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