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Human Leukemia inhibitory factor,LIF ELISA kit

  • 中文名： 人白血病抑制因子(LIF)ELISA Kit
货号: IPD-E04651h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人白血病抑制因子(LIF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的LIF含量。LIF（白血病抑制因子）是一种多功能细胞因子，属于IL-6家族，参与多种生理和病理过程。其研究机制涉及与受体结合，激活下游信号通路，调节细胞增殖、分化和免疫反应。LIF在肿瘤、炎症和干细胞培养中发挥重要作用。试剂盒检测范围为9.375 pg/mL-600 pg/mL，该产品适用于科研领域中对LIF表达水平的系统性研究，例如白血病发病机制探索、干细胞分化调控实验、炎症信号通路分析或肿瘤微环境研究，可兼容多种实验场景下的样本检测需求，包括体外细胞模型的上清液检测、动物实验中的组织样本分析以及临床前研究中的体液样本评估，为分子机制研究提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    CDF ELISA Kit; Cholinergic Differentiation Factor ELISA Kit; D factor ELISA Kit; DIA ELISA Kit; Differentiation inducing factor ELISA Kit; differentiation inhibitory activity ELISA Kit; Differentiation stimulating factor ELISA Kit; Differentiation-stimulating factor ELISA Kit; Emfilermin ELISA Kit; Hepatocyte stimulating factor III ELISA Kit; HILDA ELISA Kit; Human interleukin in DA cells ELISA Kit; Leukemia inhibitory factor ELISA Kit; LIF ELISA Kit; LIF_HUMAN ELISA Kit; Melanoma derived LPL inhibitor ELISA Kit; Melanoma-derived LPL inhibitor ELISA Kit; MLPLI ELISA Kit
  • 缩写：
    LIF
  • Uniprot No.：
    P15018
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    9.375 pg/mL-600 pg/mL
  • 灵敏度：
    2.34 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%





    Three samples of known concentration were tested twenty times on one plate to assess.





    Inter-assay Precision (Precision between assays): CV%<10%





    Three samples of known concentration were tested in twenty assays to assess.












  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human LIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.



    		SampleSerum(n=4)
    1:1Average %93
    Range %90-97

    1:2Average %103
    Range %98-106

    1:4Average %87
    Range %83-91

    1:8Average %98
    Range %92-101

  • 回收率：
    The recovery of human LIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.












    Sample TypeAverage % RecoveryRange



    Serum (n=5)9186-94



    EDTA plasma (n=4)10197-104

















  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/mlOD1OD2AverageCorrected 6002.5452.4872.5162.356 3001.6111.5961.6041.444 1501.0791.1561.1180.958 750.7300.7420.7360.576 37.50.5090.5210.5150.355 18.750.3190.3270.3230.163 9.380.2400.2320.2360.076 00.1580.1620.160  


  • 货期：
    3-5 working days

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