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产品描述：兔子黄体生成素（LH）酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LH含量。LH即促黄体生成素，是腺垂体分泌的糖蛋白激素。其生理背景在于参与调节生殖系统，对女性排卵和男性睾酮合成起关键作用。研究机制上，主要通过与靶细胞上受体结合，激活信号通路，调控生殖相关基因表达与细胞功能。试剂盒检测范围为1.56 mIU/mL-100 mIU/mL，该产品可应用于兔繁殖生物学研究、激素水平动态监测、生殖疾病模型评估或药物干预实验中LH表达变化的分析，尤其适用于卵巢、垂体等组织局部LH表达水平的检测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：LHBLutropin subunit beta ELISA kit; Lutropin beta chain ELISA kit; Luteinizing hormone subunit beta ELISA kit; LH-B ELISA kit; LSH-B ELISA kit; LSH-beta ELISA kit
缩写：LH
Uniprot No.：Q6IY74
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 mIU/mL-100 mIU/mL
灵敏度：0.39 mIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit LH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 84-95 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 89-102 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 92-104 | |||||
回收率：
| The recovery of rabbit LH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 90-104 | ||||
| EDTA plasma (n=4) | 100 | 94-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mIU/mlOD1OD2AverageCorrected 1002.8172.8392.8282.670 502.2202.2812.2512.093 251.5251.5791.5521.394 12.50.8810.8660.8740.716 6.250.5570.5690.5630.405 3.120.3900.3840.3870.229 1.560.2520.2610.2570.099 00.1570.1590.158 | ||||||
货期：3-5 working days
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