首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人溶酶体酸性脂肪酶/胆固醇酯水解酶(LIPA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的LIPA含量。LIPA 即溶酶体酸性脂肪酶，它在脂质代谢中起关键作用。当 LIPA 功能缺陷时会引发胆固醇酯和甘油三酯在溶酶体内蓄积。目前研究聚焦其参与脂质代谢的机制，探索通过调节它来改善脂质代谢紊乱相关疾病。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，该产品可用于体外研究LIPA在脂质代谢调控中的生物学功能，适用于细胞模型中酶活性相关基因调控实验、动物疾病模型的组织表达水平分析，或药物干预下LIPA表达变化的动态监测，为脂代谢相关疾病的分子机制研究及潜在治疗靶点开发提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Acid cholesteryl ester hydrolase ELISA Kit; CESD ELISA Kit; cholesterol ester hydrolase ELISA Kit; cholesterol ester storage disease ELISA Kit; Cholesteryl esterase ELISA Kit; Hydrolase deficiency ELISA Kit; LAL ELISA Kit; LAL deficiency cholesterol ester ELISA Kit; LICH_HUMAN ELISA Kit; lipA ELISA Kit; LIPA deficiency ELISA Kit; Lipase A ELISA Kit; lipase A; lysosomal acid; cholesterol esterase ELISA Kit; lysosomal acid lipase ELISA Kit; lysosomal acid lipase deficiency ELISA Kit; Lysosomal acid lipase/cholesteryl ester hydrolase ELISA Kit; Sterol esterase ELISA Kit
缩写：LIPA
Uniprot No.：P38571
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human LIPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-90 | |||||
| 1:2 | Average % | 105 | ||||
| Range % | 101-09 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 85-91 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 91-99 | |||||
回收率：
| The recovery of human LIPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 86 | 82-90 | ||||
| EDTA plasma (n=4) | 94 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.1422.1682.1552.02320001.8511.7321.7921.66010001.2641.2971.2811.1495000.8980.8870.8930.7612500.5630.5980.5810.4491250.3520.3460.3490.21762.50.2010.2060.2040.07200.1310.1330.132 | ||||||
货期：3-5 working days
×
