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Mouse Hormone-sensitive lipase(LIPE) ELISA kit

  • 中文名: 小鼠激素敏感性脂肪酶(LIPE) ELISA kit
货号: IPD-EL012975MO
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    小鼠激素敏感性脂肪酶(LIPE)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的LIPE含量。LIPE(脂酶E,激素敏感型)是一种参与脂质代谢的酶,其突变与多种疾病相关,如黄斑变性、糖尿病视网膜病变等。研究显示,LIPE在调节视网膜免疫活动方面发挥重要作用,其功能失活可能导致视力下降。此外,LIPE在脂质代谢中的异常也可能与肥胖、糖尿病等代谢性疾病有关。试剂盒检测范围为0.156 U/mL-10 U/mL,适用于基础科研中探究脂肪分解调控机制、肥胖相关代谢模型评估,以及糖尿病或代谢综合征等疾病研究中脂代谢动态变化的体外检测,为实验室提供稳定可靠的定量分析工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    Lipe ELISA Kit; Hormone-sensitive lipase ELISA Kit; HSL ELISA Kit; EC 3.1.1.79 ELISA Kit
  • 缩写:
    LIPE
  • Uniprot No.:
    P54310
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.156 U/mL-10 U/mL
  • 灵敏度:
    0.039 U/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Metabolism
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse LIPE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    85


    Range %

    81-89


    1:2

    Average %

    93


    Range %

    90-95


    1:4

    Average %

    83


    Range %

    80-86


    1:8

    Average %

    110


    Range %

    104-116


  • 回收率:

    The recovery of mouse LIPE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    89

    85-97


    EDTA plasma (n=4)

    101

    98-104


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    U/ml

    OD1

    OD2

    Average

    Corrected


    10

    2.115

    2.201

    2.158

    2.025


    5

    1.658

    1.638

    1.648

    1.515


    2.5

    1.182

    1.201

    1.192

    1.059


    1.25

    0.745

    0.738

    0.742

    0.609


    0.625

    0.418

    0.431

    0.425

    0.292


    0.312

    0.325

    0.319

    0.322

    0.189


    0.156

    0.204

    0.211

    0.208

    0.075


    0

    0.134

    0.132

    0.133





  • 货期:
    3-5 working days

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