首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人内皮脂肪酶(EL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、尿液样本中的LIPG含量。LIPG即内皮脂肪酶，它在脂质代谢中扮演重要角色。研究发现其活性与高密度脂蛋白代谢紧密相关。相关机制研究聚焦于它对脂蛋白的水解作用，影响血浆脂蛋白水平及组成，有望成为心血管疾病等脂质相关疾病治疗的潜在靶点。试剂盒检测范围为0.625 ng/mL-40 ng/mL，本产品适用于细胞模型构建、疾病动物实验或临床样本分析等科研场景，例如评估药物干预或基因编辑对EL分泌的影响，探究其在不同组织中的表达调控机制，为心血管代谢相关研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：EDL ELISA Kit; EL ELISA Kit; Endothelial cell-derived lipase ELISA Kit; Endothelial lipase ELISA Kit; Endothelial lipase precursor ELISA Kit; Lipase endothelial ELISA Kit; LIPE_HUMAN ELISA Kit; LIPG ELISA Kit; Lipoprotein lipase H ELISA Kit; PRO719 ELISA Kit
缩写：LIPG
Uniprot No.：Q9Y5X9
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, urine
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human EL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 83-102 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-102 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 89-99 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 87-108 | |||||
回收率：
| The recovery of human EL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 86-104 | ||||
| EDTA plasma (n=4) | 95 | 89-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.4152.3862.4012.271201.7021.7341.7181.588101.2041.1951.2001.07050.7300.7110.7210.5912.50.4170.4290.4230.2931.250.2960.2850.2910.1610.6250.2340.2500.2420.11200.1270.1320.130 | ||||||
货期：3-5 working days
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