首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠层连蛋白/板层素(LN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的LN含量。小鼠层连蛋白（LN）是一种细胞外基质蛋白，参与细胞粘附、迁移和细胞信号传导。其功能背景涉及多种生理过程，如胚胎发育、组织修复和肿瘤生长。研究机制主要聚焦于LN与细胞表面受体相互作用，以及其在细胞骨架形成和细胞间通讯中的作用。试剂盒检测范围为1.56 ng/mL-100 ng/mL，该产品适用于基础科研领域，如探索组织再生机制、纤维化病理模型（如肝/肺纤维化）的分子标志物动态变化，或体外培养细胞分泌LN的功能研究，为相关疾病机制解析及药物干预效果评估提供可靠工具。试剂盒支持多种生物样本类型，满足不同实验场景需求，且操作流程简便，重复性良好，适用于实验室常规检测与高通量筛选。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：LN
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse LN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 99 | ||||
| Range % | 94-102 | |||||
| 1:2 | Average % | 87 | ||||
| Range % | 84-90 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 88-96 | |||||
| 1:8 | Average % | 101 | ||||
| Range % | 97-105 | |||||
回收率：
| The recovery of mouse LN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 98 | 94-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.4072.4692.4382.281501.4721.5021.4871.330250.9090.9350.9220.76512.50.5970.5780.5880.4316.250.3640.3780.3710.2143.120.2740.2980.2860.1291.560.2090.2110.2100.05300.1580.1560.157 | ||||||
货期：3-5 working days
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