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产品描述：人赖氨酰氧化酶同源物2(LOXL2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的LOXL2含量。LOXL2 是一种赖氨酰氧化酶家族成员。其背景上，与组织纤维化、肿瘤进展等病理过程关联密切。研究机制方面，它参与调控细胞外基质交联和重构，影响细胞微环境，还会作用于相关信号通路，影响细胞增殖、迁移等，是潜在治疗靶点。试剂盒检测范围为37.5 pg/mL-2400 pg/mL，适用于科研场景中LOXL2蛋白表达水平的系统性分析，如纤维化疾病模型研究、肿瘤转移机制探索、药物干预效果评估等基础研究领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：LOR 2 ELISA Kit; LOR2 ELISA Kit; LOX L2 ELISA Kit; LOXL 2 ELISA Kit; LOXL2 ELISA Kit; LOXL2_HUMAN ELISA Kit; Lysyl oxidase homolog 2 ELISA Kit; Lysyl oxidase like 2 ELISA Kit; Lysyl oxidase like protein 2 ELISA Kit; Lysyl oxidase related 2 ELISA Kit; Lysyl oxidase related protein 2 ELISA Kit; Lysyl oxidase related protein WS9 14 ELISA Kit; Lysyl oxidase-like protein 2 ELISA Kit; Lysyl oxidase-related protein 2 ELISA Kit; Lysyl oxidase-related protein WS9-14 ELISA Kit; WS9 14 ELISA Kit
缩写：LOXL2
Uniprot No.：Q9Y4K0
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：37.5 pg/mL-2400 pg/mL
灵敏度：9.375 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human LOXL2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 81-95 | |||||
| 1:2 | Average % | 91 | ||||
| Range % | 86-97 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 91-102 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 97-107 | |||||
回收率：
| The recovery of human LOXL2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 89-99 | ||||
| EDTA plasma (n=4) | 95 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected24002.6192.5192.5692.41312001.8901.7901.8401.6846001.0271.0171.0220.8663000.5120.5020.5070.3511500.3190.3090.3140.158750.2460.2360.2410.08537.50.1990.1970.1980.04200.1560.1550.156 | ||||||
货期：3-5 working days
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