首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠脂蛋白脂酶(LPL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的LPL含量。LPL即脂蛋白脂肪酶，是脂肪代谢关键酶。它在血浆脂蛋白代谢、能量平衡等过程中发挥重要作用。研究机制方面，其能水解乳糜微粒和极低密度脂蛋白中的甘油三酯，产生游离脂肪酸供组织摄取利用，在脂代谢疾病等研究中备受关注。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于小鼠模型中脂代谢调控机制研究、心血管疾病模型评估及药物干预实验等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Lpl ELISA Kit; Lipoprotein lipase ELISA Kit; LPL ELISA Kit; EC 3.1.1.34 ELISA Kit
缩写：LPL
Uniprot No.：P11152
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse LPL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 87-93 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 88-95 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-97 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 95-103 | |||||
回收率：
| The recovery of mouse LPL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 91-97 | ||||
| EDTA plasma (n=4) | 95 | 92-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 402.4092.3482.3792.233 201.7881.7671.7781.632 101.1021.0881.0950.949 50.6910.6880.6900.544 2.50.4440.4340.4390.293 1.250.3010.3080.3050.159 0.6250.2320.2420.2370.091 00.1440.1480.146 | ||||||
货期：3-5 working days
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