首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人脂蛋白脂酶(LPL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LPL含量。LPL（脂蛋白脂肪酶）是人体脂质代谢过程中的关键靶点。它能水解血液循环中乳糜微粒和极低密度脂蛋白的甘油三酯，释放脂肪酸供组织利用。相关研究聚焦其活性调节机制，以探索改善脂代谢异常、防治心血管疾病等方面的应用。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于科研领域探究脂代谢调控机制及相关疾病研究；可用于体外研究LPL在脂质代谢异常、肥胖症发生发展中的作用，或用于评估药物、营养干预对LPL表达调控的效果，为心血管疾病及代谢综合征的机制探索提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：EC 3.1.1 ELISA Kit; EC 3.1.1.34 ELISA Kit; HDLCQ11 ELISA Kit; LIPD ELISA Kit; LIPL_HUMAN ELISA Kit; Lipoprotein lipase ELISA Kit; LPL ELISA Kit; LPL protein ELISA Kit; MGC137861 ELISA Kit
缩写：LPL
Uniprot No.：P06858
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human LPL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 90 | ||||
| Range % | 84-97 | |||||
| 1:200 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| 1:400 | Average % | 99 | ||||
| Range % | 95-104 | |||||
| 1:800 | Average % | 92 | ||||
| Range % | 88-95 | |||||
回收率：
| The recovery of human LPL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 84-96 | ||||
| EDTA plasma (n=4) | 93 | 89-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.5792.5992.5892.43510002.2642.2152.2402.0865001.7981.7711.7851.6312501.3691.3701.3701.2161250.9420.9820.9620.80862.50.5550.5690.5620.40831.250.3060.3210.3140.16000.1530.1550.154 | ||||||
货期：3-5 working days
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