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Human leucine-rich alpha-2 glycoprotein 1(LRG1) ELISA Kit

  • 中文名： 人富亮氨酸α2糖蛋白1(LRG1)ELISA Kit
货号: IPD-E12962h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人富亮氨酸α2糖蛋白1(LRG1)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、尿液、组织匀浆样本中的LRG1含量。试剂盒检测范围为0.156 μg/mL-40 μg/mL，科研人员可通过研究LRG1在心血管疾病、糖尿病肾病、肿瘤转移等病理过程中的表达动态，分析其与疾病进展的相关性，或用于药物干预实验中的生物标志物检测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    1300008B03Rik ELISA Kit; 2310031E04Rik ELISA Kit; A2GL ELISA Kit; A2GL_HUMAN ELISA Kit; HMFT1766 ELISA Kit; Leucine rich alpha 2 glycoprotein ELISA Kit; Leucine-rich alpha-2-glycoprotein 1 ELISA Kit; Leucine-rich alpha-2-glycoprotein ELISA Kit; Leucine-rich alpha-2-glycoprotein precursor ELISA Kit; LRG ELISA Kit; LRG1 ELISA Kit
  • 缩写：
    LRG1
  • Uniprot No.：
    P02750
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, urine, tissue homogenates
  • 检测范围：
    0.156 μg/mL-40 μg/mL
  • 灵敏度：
    0.156 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<12%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<15%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human LRG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    89


    Range %

    84-95


    1:2

    Average %

    90


    Range %

    85-95


    1:4

    Average %

    95


    Range %

    90-102


    1:8

    Average %

    92


    Range %

    87-97


  • 回收率：

    The recovery of human LRG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    91

    87-96


    EDTA plasma (n=4)

    95

    91-100


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    µg/ml

    OD1

    OD2

    Average




    40

    0.132

    0.134

    0.133




    10

    0.340

    0.357

    0.348




    2.5

    0.655

    0.641

    0.648




    0.625

    0.959

    0.965

    0.962




    0.156

    1.469

    1.479

    1.474




    0

    2.038

    2.063

    2.051




  • 本试剂盒所含材料：



    • A 96-well Assay plate --The 96-well plate has been pre-coated with LRG1.

    • Standard (2 x 250 μl)--Dilute the standard at dilution series, read the OD values, and then draw a standard curve.

    • Anti-human LRG1 antibody (100 x concentrate) (1 x 60 μl)

    • Antibody Diluent (1 x 10 ml) --Dilute the anti-human LRG1

    • HRP-conjugated goat-anti-mouse LRG1antibody (100 x concentrate) (1 x 120 μl) --Act as the detection antibody.

    • HRP-conjugate Diluent (1 x 20 ml) --Dilute the HRP-conjugated LRG1

  • 本试剂盒不含材料：



    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.

    • An incubator that can provide stable incubation conditions up to 37°C±5°C.

    • Centrifuge

    • Vortex

    • Squirt bottle, manifold dispenser, or automated microplate washer

    • Absorbent paper for blotting the microtiter plate


  • 货期：
    3-5 working days

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