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产品描述：人肿瘤坏死因子β(TNF-β)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的LTA含量。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于体外培养细胞模型、动物实验样本或临床前研究中TNF-β表达水平的动态监测，为科研人员探索TNF-β在炎症信号通路、免疫细胞调控或药物干预实验中的功能研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：LTalpha ELISA Kit; DAMA-25N12.13-004 ELISA Kit; DIF ELISA Kit; LT alpha ELISA Kit; LT ELISA Kit; LT-alpha ELISA Kit; Lta ELISA Kit; Lymphotoxin alpha (TNF superfamily, member 1) ELISA Kit; Lymphotoxin alpha ELISA Kit; Lymphotoxin-alpha ELISA Kit; MGC117668 ELISA Kit; OTTHUMP00000037612 ELISA Kit; OTTHUMP00000037613 ELISA Kit; TNF B ELISA Kit; TNF superfamily member 1 ELISA Kit; TNF, lymphocyte-derived ELISA Kit; TNF-beta ELISA Kit; TNFB ELISA Kit; TNFB_HUMAN ELISA Kit; TNFbeta ELISA Kit; TNFSF 1 ELISA Kit; TNFSF1 ELISA Kit; TNLG1E ELISA Kit; Tumor necrosis factor beta ELISA Kit; tumor necrosis factor ligand 1E ELISA Kit; Tumor necrosis factor ligand superfamily member 1 ELISA Kit; tumor necrosis factor superfamily member 1 ELISA Kit
缩写：LTA
Uniprot No.：P01374
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TNF-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 83-99 | |||||
| 1:2 | Average % | 87 | ||||
| Range % | 84-93 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 90-104 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-102 | |||||
回收率：
| The recovery of human TNF-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-100 | ||||
| EDTA plasma (n=4) | 97 | 90-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10001.8231.9111.8671.7795001.3151.3761.3461.2582500.8070.8160.8120.7241250.5330.5730.5530.46562.50.3240.3080.3160.22831.20.2590.2460.2530.16515.60.1590.1630.1610.07300.0860.0900.088 | ||||||
货期：3-5 working days
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