首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠淋巴毒素β(LTB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LTB含量。靶点研究是药物研发的关键，旨在发现疾病过程中的特定分子或细胞结构，通过药物相互作用产生治疗效果。研究机制涉及基因组学、蛋白质组学、生物信息学和高通量筛选，旨在精准识别疾病靶点，开发高效药物。试剂盒检测范围为12.5 pg/mL-800 pg/mL，可满足科研场景中对LTB的定量分析需求，如免疫相关疾病模型研究、炎症信号通路探索或药物干预实验中生物标志物动态监测，为探究LTB在免疫调控中的功能机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ltb; Tnfc; Tnfsf3; Lymphotoxin-beta; LT-beta; Tumor necrosis factor C; TNF-C; Tumor necrosis factor ligand superfamily member 3
缩写：LTB
Uniprot No.：P41155
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：12.5 pg/mL-800 pg/mL
灵敏度：3.12 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse LTB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 86-94 | |||||
| 1:2 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 84-93 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 94-102 | |||||
回收率：
| The recovery of mouse LTB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-99 | ||||
| EDTA plasma (n=4) | 104 | 100-108 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected8002.7042.5272.6162.4664002.0472.2062.1271.9772001.4151.5041.4601.3101000.7040.7350.7200.570500.3940.4190.4070.257250.2910.2790.2850.13512.50.2130.2050.2090.05900.1540.1450.150 | ||||||
货期：3-5 working days
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