首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠肿瘤坏死因子β(TNF-β)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LTA含量。LTA即淋巴毒素-α，是TNF超家族成员，在免疫调节、炎症反应等方面扮演关键角色。其研究机制主要围绕与受体结合激活下游信号通路，影响细胞存活、分化及炎性因子释放，对自身免疫疾病、肿瘤等疾病的病理过程研究有重要意义。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，本试剂盒适用于小鼠模型中的炎症机制研究、肿瘤免疫微环境分析、药物干预效果评估等科研领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Lta ELISA Kit; Tnfb ELISA Kit; Tnfsf1 ELISA Kit; Lymphotoxin-alpha ELISA Kit; LT-alpha ELISA Kit; TNF-beta ELISA Kit; Tumor necrosis factor ligand superfamily member 1 ELISA Kit
缩写：LTA
Uniprot No.：P09225
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：18.75 pg/mL-1200 pg/mL
灵敏度：4.7 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNF-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 90-100 | |||||
| 1:2 | Average % | 105 | ||||
| Range % | 100-110 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 88-100 | |||||
| 1:8 | Average % | 84 | ||||
| Range % | 80-88 | |||||
回收率：
| The recovery of mouse TNF-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
| EDTA plasma (n=4) | 90 | 85-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected12002.6742.7522.7132.5346002.2542.1762.2152.0363001.4351.4041.4201.2411500.8050.8390.8220.643750.4980.5240.5110.33237.50.3560.3380.3470.16818.750.2540.2390.2470.06800.1750.1820.179 | ||||||
货期：3-5 working days
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