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产品描述：猪乳铁传递蛋白/乳铁蛋白(LF/LTF)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的LTF含量。靶点研究旨在发现和验证药物与生物体内特定分子相互作用的目标位点，如基因、受体、酶等。通过分析这些靶点，研究人员可以揭示疾病机制，并开发针对性强的新药。研究机制包括遗传技术、计算技术、分子技术等，旨在筛选和验证潜在的药物靶点。试剂盒检测范围为0.156 μg/mL-10 μg/mL，适用于动物免疫学研究、疾病模型建立（如乳腺炎或肠道炎症研究）、乳制品营养成分分析等科研场景，为探索乳铁蛋白在猪生理功能、病原体防御机制及营养代谢中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：LTF ELISA Kit; Lactotransferrin ELISA Kit; Lactoferrin ELISA Kit; EC 3.4.21.- ELISA Kit
缩写：LTF
Uniprot No.：P14632
种属：Sus scrofa (Pig)
样本类型：serum, plasma
检测范围：0.156 μg/mL-10 μg/mL
灵敏度：0.078 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig LF/LTFin various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:1 | Average % | 103 | |
| Range % | 99-107 | ||
| 1:2 | Average % | 85 | |
| Range % | 81-89 | ||
| 1:4 | Average % | 98 | |
| Range % | 94-102 | ||
| 1:8 | Average % | 91 | |
| Range % | 87-95 |
回收率：
| The recovery of pig LF/LTF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 91-102 | ||||
| EDTA plasma (n=4) | 86 | 80-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2Average 100.1350.1370.136 50.2240.2150.220 2.50.3480.3530.351 1.250.5760.5680.572 0.6251.0061.0211.014 0.3121.5871.6711.629 0.1561.9981.8541.926 02.3542.2282.291 | ||||||
货期：3-5 working days
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