首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人溶菌酶(LZM)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、尿液样本中的LZM含量。LZM即溶菌酶，是一种天然抗菌物质，具有破坏细菌细胞壁的功能。它能特异性水解细胞壁中肽聚糖的β-1,4糖苷键，使细菌裂解死亡。在医药、食品等领域应用广泛，有抗菌、抗病毒、消炎等作用，相关研究持续探索其更多功效与应用。试剂盒检测范围为3.12 ng/mL-200 ng/mL，为研究溶菌酶在感染性疾病模型中的表达模式、评估组织局部免疫状态、探索泌尿系统防御机制等科研方向提供可靠工具。实验操作兼容常规酶标仪平台，适用于细胞实验、动物模型及生物标志物筛选等基础研究领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：1 4 beta N acetylmuramidase C ELISA Kit; 1 ELISA Kit; 4-beta-N-acetylmuramidase C ELISA Kit; EC 3.2.1.17 ELISA Kit; LYSC_HUMAN ELISA Kit; Lysosyme ELISA Kit; Lysozyme (renal amyloidosis) ELISA Kit; Lysozyme C ELISA Kit; Lysozyme C precursor ELISA Kit; LYZ ELISA Kit; LZM ELISA Kit; Renal amyloidosis ELISA Kit
缩写：LZM
Uniprot No.：P61626
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, urine
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human LZM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 89 | ||||
| Range % | 83-97 | |||||
| 1:40 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:80 | Average % | 96 | ||||
| Range % | 92-101 | |||||
| 1:160 | Average % | 92 | ||||
| Range % | 88-98 | |||||
回收率：
| The recovery of human LZM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 98 | 90-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected2002.7672.7992.7832.6251002.1702.1892.1802.022501.4751.4991.4871.329250.8290.8650.8470.68912.50.5480.5070.5280.3706.250.3690.3400.3550.1973.120.2830.2620.2730.11500.1590.1570.158 | ||||||
货期：3-5 working days
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