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产品描述：人Ly6/PLAUR结构域蛋白1(LYPD1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的LYPD1含量。试剂盒检测范围为25 pg/mL-1600 pg/mL，适用于基础研究领域，可用于体外评估不同生理或病理条件下（如癌症模型、神经退行性疾病模型）生物样本中LYPD1的表达水平变化，为探索其分子机制及功能研究提供定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FLJ41033 ELISA Kit; LY6/PLAUR domain containing 1 ELISA Kit; Ly6/PLAUR domain-containing protein 1 ELISA Kit; Lypd1 ELISA Kit; LYPD1_HUMAN ELISA Kit; LYPDC1 ELISA Kit; MGC29643 ELISA Kit; PHTS ELISA Kit; Putative HeLa tumor suppressor ELISA Kit
缩写：LYPD1
Uniprot No.：Q8N2G4
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human LYPD1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 83-91 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-104 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 82-90 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 90-96 | |||||
回收率：
| The recovery of human LYPD1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 98 | 92-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected16002.2432.2302.2372.1008001.4851.4721.4791.3424000.9240.9840.9540.8172000.5910.6170.6040.4671000.4330.4260.4300.293500.3370.3170.3270.190250.2290.2370.2330.09600.1360.1380.137 | ||||||
货期：3-5 working days
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