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产品描述：人髓鞘相关糖蛋白(MAG)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、脑脊液（CSF）样本中的MAG含量。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于神经科学基础研究、疾病模型构建、药物开发中生物标志物检测等场景，尤其可为多发性硬化症、吉兰巴雷综合征等疾病的分子机制探索提供定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：GMA ELISA Kit; MAG ELISA Kit; MAG_HUMAN ELISA Kit; Myelin associated glycoprotein ELISA Kit; Myelin-associated glycoprotein ELISA Kit; S MAG ELISA Kit; S-MAG ELISA Kit; Sialic acid binding Ig like lectin 4A ELISA Kit; Sialic acid binding immunoglobulin like lectin 4A ELISA Kit; Siglec 4a ELISA Kit; Siglec-4a ELISA Kit; SIGLEC4A ELISA Kit; SPG75 ELISA Kit
缩写：MAG
Uniprot No.：P20916
种属：Homo sapiens (Human)
样本类型：serum, plasma, cerebrospinal fluid (CSF)
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MAG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 89-101 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 84-96 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-95 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 90-102 | |||||
回收率：
| The recovery of human MAG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 89-101 | ||||
| EDTA plasma (n=4) | 98 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected401.7541.7211.7381.615201.3951.4221.4091.286100.8720.9110.8920.76950.5510.5550.5530.4302.50.3650.3480.3570.2341.250.2310.2420.2370.1140.6250.1870.1840.1860.06300.1240.1220.123 | ||||||
货期：3-5 working days
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