首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人黑色素瘤相关抗原D2(MAGED2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的MAGED2含量。MAGED2基因是MAGE家族成员之一，位于X染色体上，与X连锁智力障碍和某些癌症相关。其蛋白在细胞周期调节中发挥作用，可变剪接导致多个转录本变体。研究发现，MAGED2蛋白具有抗病毒功能，新冠病毒主蛋白酶可切割MAGED2蛋白，使其失去抗病毒作用。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，本产品可广泛应用于肿瘤分子机制研究、抗癌药物体外筛选及肿瘤相关生物标志物的表达水平分析等科研领域，为探索MAGED2在肿瘤微环境调控、转移潜能评估等方向提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：11B6 ELISA Kit; BCG 1 ELISA Kit; BCG-1 ELISA Kit; Breast cancer-associated gene 1 protein ELISA Kit; HCA10 ELISA Kit; Hepatocellular carcinoma-associated protein JCL-1 ELISA Kit; JCL 1 ELISA Kit; MAGD2_HUMAN ELISA Kit; MAGE D ELISA Kit; MAGE D2 antigen ELISA Kit; MAGE-D2 antigen ELISA Kit; MAGED2 ELISA Kit; Melanoma antigen family D 2 ELISA Kit; Melanoma associated antigen D2 ELISA Kit; Melanoma-associated antigen D2 ELISA Kit
缩写：MAGED2
Uniprot No.：Q9UNF1
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MAGED2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 89 | |||||
| Range % | 83-94 | ||||||
| 1:2 | Average % | 97 | |||||
| Range % | 92-101 | ||||||
| 1:4 | Average % | 93 | |||||
| Range % | 88-97 | ||||||
| 1:8 | Average % | 107 | |||||
| Range % | 102-111 | ||||||
回收率：
| The recovery of human MAGED2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 105 | 102-108 | |||||
| EDTA plasma (n=4) | 96 | 91-98 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected20002.2382.3342.2862.09010001.4151.5271.4711.2755000.8810.9320.9070.7112500.5600.5730.5670.3711250.3790.3810.3800.18462.50.2920.2840.2880.09231.250.2470.2520.2500.05400.1950.1960.196 | |||||||
货期：3-5 working days
×
