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产品描述：人单胺氧化酶(MAO)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MAOA含量。MAOA（单胺氧化酶A）是一种位于X染色体上的基因，编码的酶参与大脑中神经递质的代谢。研究显示，MAOA基因与暴力行为、抑郁等心理疾病有关，其变异可能影响个体对情绪和冲动的控制。研究机制主要涉及MAOA基因对神经递质代谢的影响，进而影响大脑功能和行为表现。试剂盒检测范围为0.9 IU/mL-60 IU/mL，该产品适用于科研领域对MAO活性的动态监测，例如神经退行性疾病模型研究、精神类药物代谢机制探索或心血管疾病相关的氧化应激分析，也可用于评估不同组织样本中MAO的分布与表达水平，为生物医学研究提供可靠的工具支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：MAOA; Amine oxidase [flavin-containing] A; Monoamine oxidase type A; MAO-A
缩写：MAOA
Uniprot No.：P21397
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.9 IU/mL-60 IU/mL
灵敏度：0.225 IU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MAO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 93 | ||||
| Range % | 91-95 | |||||
| 1:10 | Average % | 104 | ||||
| Range % | 99-107 | |||||
| 1:20 | Average % | 94 | ||||
| Range % | 89-97 | |||||
| 1:40 | Average % | 98 | ||||
| Range % | 92-103 | |||||
回收率：
| The recovery of human MAO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-97 | ||||
| EDTA plasma (n=4) | 95 | 91-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| IU/mlOD1OD2AverageCorrected602.7892.6992.7442.621302.1402.1222.1312.008151.4641.5021.4831.3607.50.7980.8610.8300.7073.750.4170.4230.4200.2971.80.3400.3280.3340.2110.90.2310.2220.2270.10400.1240.1220.123 | ||||||
货期：3-5 working days
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