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产品描述：人微管相关蛋白1A/1B轻链3B(MAP1LC3B)酶联免疫试剂盒检测血清、血浆、组织匀浆、细胞裂解物样本中的MAP1LC3B含量。MAP1LC3B是重要靶点。它参与自噬过程，在细胞内物质降解和再利用等方面发挥关键作用。在研究机制上，其表达水平变化、修饰状态等影响自噬活性，进而与肿瘤、神经退行性疾病等多种疾病的发生发展相关，是潜在诊疗靶点。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，本产品适用于细胞自噬机制研究、药物干预效果评估及疾病模型构建等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ATG8F ELISA Kit; Autophagy-related protein LC3 B ELISA Kit; Autophagy-related ubiquitin-like modifier LC3 B ELISA Kit; LC3B ELISA Kit; LC3II ELISA Kit; MAP1 light chain 3 like protein 2 ELISA Kit; MAP1 light chain 3-like protein 2 ELISA Kit; MAP1A/1BLC3 ELISA Kit; MAP1A/MAP1B LC3 B ELISA Kit; MAP1A/MAP1B light chain 3 B ELISA Kit; MAP1ALC3 ELISA Kit; MAP1LC3B a ELISA Kit; Map1lc3b ELISA Kit; Microtubule associated protein 1 light chain 3 beta ELISA Kit; Microtubule-associated protein 1 light chain 3 beta ELISA Kit; Microtubule-associated proteins 1A/1B light chain 3B ELISA Kit; MLP3B_HUMAN ELISA Kit
缩写：MAP1LC3B
Uniprot No.：Q9GZQ8
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：18.75 pg/mL-1200 pg/mL
灵敏度：4.67 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MAP1LC3B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:1 | Average % | 84 | |
| Range % | 80-88 | ||
| 1:2 | Average % | 97 | |
| Range % | 92-101 | ||
| 1:4 | Average % | 89 | |
| Range % | 83-94 | ||
| 1:8 | Average % | 90 | |
| Range % | 85-98 |
回收率：
| The recovery of human MAP1LC3B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 104 | 99-110 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 12002.1532.0012.0771.872 6001.5691.6031.5861.381 3001.0671.0161.0420.837 1500.7130.6860.7000.495 750.4640.4310.4480.243 37.50.3310.3570.3440.139 18.750.2570.2720.2650.060 00.1980.2110.205 | ||||||
货期：3-5 working days
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