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Human myelin basic protein,MBP ELISA Kit

  • 中文名： 人髓磷脂碱性蛋白(MBP)ELISA Kit
货号: IPD-E08283h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人髓磷脂碱性蛋白(MBP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MBP含量。MBP即髓鞘碱性蛋白，在中枢神经系统髓鞘形成与维持中起关键作用。其异常表达或修饰与多发性硬化症等脱髓鞘疾病密切相关。研究聚焦于MBP与免疫细胞相互作用机制，以及其在髓鞘损伤和修复过程中的信号通路，以探寻潜在治疗靶点。试剂盒检测范围为7.8 ng/ml- 500ng/ml，可用于探索脱髓鞘疾病的分子机制、评估药物对髓鞘修复的干预效果，或结合动物模型研究脑损伤后的病理进程本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    GDB ELISA Kit; Golli MBP ELISA Kit; Golli MBP; myelin basic protein ELISA Kit; Hemopoietic MBP ELISA Kit; HMBPR ELISA Kit; HUGO ELISA Kit; MBP ELISA Kit; MBP_CAVPO ELISA Kit; MBP_HUMAN ELISA Kit; MGC99675 ELISA Kit; MLD ELISA Kit; Myelin A1 protein ELISA Kit; Myelin A1 Protein; basic ELISA Kit; Myelin basic protein ELISA Kit; Myelin Deficient ELISA Kit; Myelin membrane encephalitogenic protein ELISA Kit; OTTHUMP00000163776 ELISA Kit; OTTHUMP00000174387 ELISA Kit; OTTHUMP00000174388 ELISA Kit; SHI ELISA Kit; Shiverer ELISA Kit; SP ELISA Kit
  • 缩写：
    MBP
  • Uniprot No.：
    P02686
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    7.8 ng/ml- 500ng/ml
  • 灵敏度：
    1.95 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human MBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    101


    Range %

    90-108


    1:2

    Average %

    97


    Range %

    91-105


    1:4

    Average %

    95


    Range %

    92-100


    1:8

    Average %

    93


    Range %

    86-98


  • 回收率：

    The recovery of human MBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    98

    94-102


    EDTA plasma (n=4)

    96

    91-100


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    500

    2.796

    2.634

    2.715

    2.544


    250

    1.956

    1.867

    1.911

    1.740


    125

    1.197

    1.128

    1.163

    0.991


    62.5

    0.674

    0.639

    0.656

    0.485


    31.2

    0.476

    0.461

    0.468

    0.297


    15.6

    0.331

    0.343

    0.337

    0.166


    7.8

    0.249

    0.254

    0.251

    0.080


    0

    0.168

    0.175

    0.172




  • 货期：
    3-5 working days

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