首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠金属硫蛋白(MT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MT含量。试剂盒检测范围为0.156 ng/mL-10 ng/mL，该产品适用于多种生物样本类型，为研究MT在重金属暴露模型中的动态变化、金属代谢相关疾病的分子机制以及氧化应激调控网络提供高效工具，可应用于环境毒理学、基础医学及分子生物学等领域，助力科研人员深入探索MT在生理及病理条件下的功能与调控途径。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Mt1 ELISA Kit; Mt1a ELISA Kit; Metallothionein-1 ELISA Kit; MT-1 ELISA Kit; Metallothionein-I ELISA Kit; MT-I ELISA Kit
缩写：MT
Uniprot No.：P02803
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-89 | |||||
| 1:2 | Average % | 103 | ||||
| Range % | 99-110 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 85-94 | |||||
回收率：
| The recovery of rat MT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 91-99 | ||||
| EDTA plasma (n=4) | 93 | 88-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.9372.8322.8852.71852.0481.9191.9841.8172.51.2211.2741.2481.0811.250.7930.8320.8130.6460.6250.5180.5410.5300.3630.3120.4070.3990.4030.2360.1560.2390.2210.2300.06300.1720.1610.167 | ||||||
货期：3-5 working days
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