Rat Mucin-5 subtype AC(MUC5AC)ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E16223r

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：大鼠粘蛋白/粘液素5AC(MUC5AC)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的MUC5AC含量。MUC5AC 是一种黏蛋白。它在呼吸道等部位发挥重要作用，其异常表达与哮喘、慢性阻塞性肺疾病等多种呼吸系统疾病密切相关。研究机制方面，主要聚焦于调控其表达、分泌的信号通路，以探索通过干预这些通路来治疗相关疾病的方法。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，本试剂盒适用于基础科研领域，例如研究呼吸道疾病模型（如哮喘、慢性阻塞性肺病）中黏液分泌调控机制，评估胃肠道黏膜屏障功能变化，或探索药物、环境因素对黏液代谢通路的影响，为相关生物标志物研究及分子机制解析提供可靠工具。适用于实验室常规检测及高通量筛查需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：MUC5AC
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat MUC5AC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	94
	Range %	87-97
1:10	Average %	97
	Range %	92-102
1:20	Average %	92
	Range %	88-97
1:40	Average %	95
	Range %	89-100

回收率：

The recovery of rat MUC5AC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	85-95
EDTA plasma (n=4)	99	95-106

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/mlOD1OD2AverageCorrected 40002.1792.2542.2172.070 20001.6141.6071.6111.464 10001.2141.1561.1851.038 5000.8640.8500.8570.710 2500.5290.5640.5470.400 1250.3350.3160.3260.179 62.50.2250.2330.2290.082 00.1460.1480.147