| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TCP1 |
| Uniprot No | P48643 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-541aa |
| 氨基酸序列 | MASMGTLAFDEYGRPFLIIKDQDRKSRLMGLEALKSHIMAAKAVANTMRT SLGPNGLDKMMVDKDGDVTVTNDGATILSMMDVDHQIAKLMVELSKSQDD EIGDGTTGVVVLAGALLEEAEQLLDRGIHPIRIADGYEQAARVAIEHLDK ISDSVLVDIKDTEPLIQTAKTTLGSKVVNSCHRQMAEIAVNAVLTVADME RRDVDFELIKVEGKVGGRLEDTKLIKGVIVDKDFSHPQMPKKVEDAKIAI LTCPFEPPKPKTKHKLDVTSVEDYKALQKYEKEKFEEMIQQIKETGANLA ICQWGFDDEANHLLLQNNLPAVRWVGGPEIELIAIATGGRIVPRFSELTA EKLGFAGLVQEISFGTTKDKMLVIEQCKNSRAVTIFIRGGNKMIIEEAKR SLHDALCVIRNLIRDNRVVYGGGAAEISCALAVSQEADKCPTLEQYAMRA FADALEVIPMALSENSGMNPIQTMTEVRARQVKEMNPALGIDCLHKGTND MKQQHVIETLIGKKQQISLATQMVRMILKIDDIRKPGESEE |
| 预测分子量 | 85 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TCP1重组蛋白的3篇参考文献示例(注：以下为虚构示例，实际文献需通过学术数据库查询)：
1. **文献名称**：*Structural Insights into the Chaperonin TCP1 Complex in Protein Folding*
**作者**：Frydman, J. et al.
**摘要**：该研究通过冷冻电镜解析了TCP1复合体的三维结构，揭示了其在协助细胞周期相关蛋白(如β-tubulin)正确折叠中的分子机制，并证明其ATP依赖的构象变化对功能至关重要。
2. **文献名称**：*Functional Analysis of Recombinant TCP1 in Cancer Cell Proliferation*
**作者**：Kitagawa, M. et al.
**摘要**：通过体外重组TCP1蛋白实验，发现其过表达显著促进癌细胞增殖，并调控细胞周期蛋白的稳定性，提示TCP1可能作为肿瘤治疗的潜在靶点。
3. **文献名称**：*TCP1-Mediated Protein Quality Control in Neurodegenerative Diseases*
**作者**：Grantham, J. et al.
**摘要**：研究表明重组TCP1蛋白可减轻错误折叠蛋白在神经元中的聚集，通过体外实验验证了其在阿尔茨海默病模型中的保护作用，为神经退行性疾病机制提供新视角。
4. **文献名称**：*ATPase Activity of Recombinant TCP1 and Its Role in Actin Assembly*
**作者**：Kubota, H. et al.
**摘要**：纯化并表征了重组TCP1的ATP酶活性，发现其通过调控细胞骨架蛋白(如actin)的组装影响细胞迁移，揭示了TCP1在细胞运动中的功能。
建议通过PubMed、Google Scholar等平台以关键词“TCP1 chaperonin”、“TCP1 recombinant protein”检索真实文献。
**Background of TCP1 Recombinant Protein**
TCP1 (T-complex protein 1), also known as CCT1 (Chaperonin Containing TCP-1 subunit 1), is a critical component of the eukaryotic chaperonin complex CCT/TRiC (Tailless Complex Polypeptide 1 Ring Complex). This molecular chaperone plays a vital role in assisting the folding of newly synthesized polypeptides, particularly cytoskeletal proteins such as actin and tubulin, ensuring their proper tertiary structure and functional integrity. The CCT complex consists of two stacked rings, each containing eight distinct subunits (CCT1 to CCT8), with TCP1 representing the alpha subunit.
Recombinant TCP1 protein is produced using biotechnological systems (e.g., *E. coli*, yeast, or mammalian cell lines*) to express the purified protein *in vitro*. This engineered approach allows researchers to study TCP1’s structural and functional properties in isolation, bypassing the complexity of the native CCT complex. Recombinant TCP1 retains chaperone-like activity, enabling investigations into its ATP-dependent folding mechanisms, substrate interactions, and role in cellular processes like cell cycle regulation, stress response, and protein quality control.
Studies on TCP1 have highlighted its involvement in diseases, including cancer and neurodegenerative disorders, where misfolded proteins accumulate. Recombinant TCP1 serves as a tool for drug discovery, structural biology (e.g., cryo-EM studies), and understanding chaperonin dysfunction in pathology. Its application extends to *in vitro* protein refolding assays and exploring therapeutic strategies targeting protein misfolding.
Overall, TCP1 recombinant protein bridges molecular biology and translational research, offering insights into chaperone-mediated proteostasis and potential clinical interventions.
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