| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TCF4 |
| Uniprot No | P15884 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-507aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMKFKQCR CSDTGLCCLD HEGKAEVYAP SASTADYNRD SPGYPSSKPA TSTFPSSFFM QDGHHSSDPW SSSSGMNQPG YAGMLGNSSH IPQSSSYCSL HPHERLSYPS HSSADINSSL PPMSTFHRSG TNHYSTSSCT PPANGTDSIM ANRGSGAAGS SQTGDALGKA LASIYSPDHT NNSFSSNPST PVGSPPSLSA GTAVWSRNGG QASSSPNYEG PLHSLQSRIE DRLERLDDAI HVLRNHAVGP STAMPGGHGD MHGIIGPSHN GAMGGLGSGY GTGLLSANRH SLMVGTHRED GVALRGSHSL LPNQVPVPQL PVQSATSPDL NPPQDPYRGM PPGLQGQSVS SGSSEIKSDD EGDENLQDTK SSEDKKLDDD KKDIKSITSN NDDEDLTPEQ KAEREKERRM ANNARERLRV RDINEAFKEL GRMVQLHLKS DKPQTKLLIL HQAVAVILSL EQQVRERNLN PKAACLKRRE EEKVSSEPPP LSLAGPHPGM GDASNHMGQM |
| 预测分子量 | 57 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TCF4重组蛋白的3篇参考文献，包含文献名称、作者及摘要内容概括：
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1. **文献名称**: *Structural and Functional Analysis of the TCF4-β-catenin Complex*
**作者**: Valenta T. et al.
**摘要**: 该研究解析了重组TCF4蛋白与β-catenin的复合物结构，揭示了二者在Wnt信号通路中的相互作用机制，并验证了重组TCF4在体外调控靶基因转录的功能。
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2. **文献名称**: *Production of Recombinant Human TCF4 for Epigenetic Studies*
**作者**: Liu Y. et al.
**摘要**: 文章描述了利用大肠杆菌表达系统高效制备重组人源TCF4蛋白的方法，优化了纯化条件，并证明其可用于染色质免疫共沉淀(ChIP)等表观遗传学研究。
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3. **文献名称**: *TCF4 Recombinant Protein Modulates Neuronal Differentiation in vitro*
**作者**: Forrest M.P. et al.
**摘要**: 通过体外实验发现，重组TCF4蛋白可促进神经干细胞向神经元分化，提示其在神经发育中的关键作用，为研究TCF4突变相关精神疾病提供了工具。
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**备注**：若需具体文献来源或补充更多研究，可进一步提供数据库(如PubMed ID)或研究方向细化。
TCF4 (T-cell factor 4), also known as transcription factor 7-like 2 (TCF7L2), is a member of the TCF/LEF family of transcription factors that plays a critical role in the Wnt/β-catenin signaling pathway. This pathway is essential for embryonic development, tissue homeostasis, and stem cell regulation. TCF4 acts as a nuclear mediator by binding to β-catenin, which translocates to the nucleus upon Wnt activation, forming a complex that drives the expression of target genes involved in cell proliferation, differentiation, and survival. Dysregulation of TCF4 is linked to various diseases, including cancers (e.g., colorectal, breast) and neurodevelopmental disorders like Pitt-Hopkins syndrome, a rare genetic condition characterized by severe intellectual disability and developmental delays.
Recombinant TCF4 proteins are engineered in vitro using expression systems (e.g., bacterial, mammalian) to produce purified, functional forms of the protein for research and therapeutic applications. These proteins often include specific domains, such as the N-terminal β-catenin-binding region or the C-terminal DNA-binding domain, enabling studies on molecular interactions, structural analysis, and pathway modulation. Researchers utilize recombinant TCF4 to investigate its role in Wnt signaling dynamics, screen for inhibitors or activators of the pathway, and model disease mechanisms. For example, in cancer research, it helps identify therapeutic targets to disrupt oncogenic Wnt signaling. In neuroscience, it aids in understanding how TCF4 mutations impair neuronal development. The production of recombinant TCF4 ensures standardized, high-quality material for advancing both basic science and drug discovery efforts.
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