| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TCEAL3 |
| Uniprot No | Q969E4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-200aa |
| 氨基酸序列 | MEKPYNKNEG NLENEGKPED EVEPDDEGKS DEEEKPDVEG KTECEGKRED EGEPGDEGQL EDEGSQEKQG RSEGEGKPQG EGKPASQAKP ESQPRAAEKR PAEDYVPRKA KRKTDRGTDD SPKDSQEDLQ ERHLSSEEMM RECGDVSRAQ EELRKKQKMG GFHWMQRDVQ DPFAPRGQRG VRGVRGGGRG QRGLHDIPYL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3条关于TCEAL3重组蛋白的示例参考文献(注：部分信息为示例，实际文献需通过学术数据库验证)：
1. **文献名称**：*TCEAL3 modulates ovarian cancer cell proliferation through transcriptional regulation of cell cycle genes*
**作者**：Smith, J. et al. (2020)
**摘要**：研究利用重组TCEAL3蛋白揭示其在卵巢癌细胞中的功能，发现其通过抑制c-Myc介导的转录活性调控细胞周期蛋白表达，抑制肿瘤生长。
2. **文献名称**：*Structural characterization of recombinant TCEAL3 and its interaction with nuclear receptors*
**作者**：Johnson, R.B. et al. (2018)
**摘要**：通过重组表达纯化人源TCEAL3蛋白，结合X射线晶体学解析其结构，发现其C端结构域与核受体(如ERα)存在特异性结合，提示其在激素信号通路中的作用。
3. **文献名称**：*TCEAL3 as a potential biomarker in Wnt/β-catenin signaling pathway*
**作者**：Lee, H. et al. (2021)
**摘要**：重组TCEAL3蛋白实验表明，其通过竞争性结合β-catenin抑制Wnt信号通路活性，在结直肠癌细胞中具有抑癌功能，可能作为治疗靶点。
4. **文献名称**：*Proteomic analysis of TCEAL3 recombinant protein interactions in neurodegenerative models*
**作者**：Patel, S. et al. (2019)
**摘要**：利用重组TCEAL3进行蛋白质互作组学研究，发现其与RNA结合蛋白相互作用，可能参与阿尔茨海默病中tau蛋白异常磷酸化的调控。
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**注意事项**：
- 以上为模拟文献，实际研究需查询PubMed、Web of Science等数据库获取真实信息。
- TCEAL3(Transcription Elongation Factor A Like 3)研究多聚焦于其在癌症、转录调控及信号转导中的作用，重组蛋白常用于功能验证和互作机制研究。
- 推荐检索关键词：**TCEAL3 recombinant protein function/structure/cancer**。
**Background of TCEAL3 Recombinant Protein**
TCEAL3 (Transcription Elongation Factor A protein-Like 3) is a member of the TCEAL family, a group of nuclear proteins implicated in transcriptional regulation and chromatin remodeling. This protein is encoded by the *TCEAL3* gene located on the X chromosome and is characterized by a conserved TEA DNA-binding domain, suggesting its potential role in modulating gene expression by interacting with transcriptional machinery or chromatin-associated complexes. While the precise molecular function of TCEAL3 remains under investigation, studies suggest it may act as a tumor suppressor or regulatory factor in cellular processes such as proliferation, differentiation, and apoptosis.
TCEAL3 expression is tissue-specific, with higher levels observed in reproductive and immune tissues. Dysregulation of TCEAL3 has been linked to cancers, including ovarian and breast cancers, where its downregulation correlates with poor prognosis. These observations highlight its potential relevance in oncogenesis and cellular homeostasis.
Recombinant TCEAL3 protein is engineered for *in vitro* studies to elucidate its biochemical properties, interactome, and mechanisms. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), the recombinant protein is often tagged for purification and detection. Its applications span structural studies, binding assays (e.g., DNA-protein or protein-protein interactions), and functional analyses to explore its role in epigenetic regulation or signaling pathways.
Current research focuses on clarifying TCEAL3’s contribution to transcriptional networks and its therapeutic potential as a biomarker or target in cancer. The availability of recombinant TCEAL3 accelerates mechanistic studies, offering insights into its physiological and pathological roles. Further work is needed to define its precise molecular partners and regulatory cascades, which could unlock novel strategies for disease intervention.
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