| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TBPL1 |
| Uniprot No | P62380 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-186aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMDADSDV ALDILITNVV CVFRTRCHLN LRKIALEGAN VIYKRDVGKV LMKLRKPRIT ATIWSSGKII CTGATSEEEA KFGARRLARS LQKLGFQVIF TDFKVVNVLA VCNMPFEIRL PEFTKNNRPH ASYEPELHPA VCYRIKSLRA TLQIFSTGSI TVTGPNVKAV ATAVEQIYPF VFESRKEIL |
| 预测分子量 | 23 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TBPL1重组蛋白研究的示例参考文献(注：部分文献为模拟示例，实际引用需核实原文)：
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1. **文献名称**: "Functional Analysis of TBPL1 in Transcriptional Regulation During Mouse Oogenesis"
**作者**: Tanaka, K. et al.
**摘要**: 本研究利用重组TBPL1蛋白，在小鼠卵母细胞中揭示了其通过替代TBP参与非经典启动子结合的转录调控机制，证实了TBPL1在生殖细胞特异性基因表达中的关键作用。
2. **文献名称**: "Structural Basis for the Selective Interaction of TBPL1 with TATA-Less Promoters"
**作者**: Wang, H. et al.
**摘要**: 通过重组TBPL1蛋白的晶体结构解析，发现其DNA结合域与TBP存在构象差异，解释了TBPL1偏好结合TATA-less启动子的分子机制，为生殖发育相关基因调控提供结构依据。
3. **文献名称**: "Recombinant TBPL1 Production in Insect Cells and Its Role in Zebrafish Embryogenesis"
**作者**: Li, Y. et al.
**摘要**: 报道了利用杆状病毒-昆虫细胞系统高效表达重组TBPL1蛋白的方法，并通过胚胎显微注射实验证明TBPL1缺失导致斑马鱼早期胚胎发育停滞，强调了其进化保守功能。
4. **文献名称**: "TBPL1 Synergizes with GATA4 to Activate Oocyte-Specific Gene Expression"
**作者**: Chen, R. et al.
**摘要**: 利用重组TBPL1和GATA4蛋白的体外共孵育实验，结合ChIP-seq分析，揭示了二者在卵母细胞中协同调控靶基因的分子通路，为生殖细胞转录网络研究提供新视角。
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建议通过PubMed或Google Scholar搜索以下关键词获取真实文献：
`TBPL1 recombinant protein expression`、`TBPL1 transcriptional mechanism`、`TBPL1 germ cell development`。
**Background of TBPL1 Recombinant Protein**
TBPL1 (TATA-box-binding protein-like 1), also known as TRF3 or TBP2. is a member of the TBP family of transcription factors, which play critical roles in regulating gene expression by binding to core promoter regions. Unlike its well-studied homolog TBP (TATA-box-binding protein), TBPL1 exhibits distinct functional and expression patterns. It is primarily expressed in oocytes, early embryos, and testis, suggesting specialized roles in germ cell development and embryogenesis.
TBPL1 lacks the conserved N-terminal domain of TBP but retains the C-terminal domain responsible for DNA binding and interaction with general transcription factors. It binds to TATA-less promoters and facilitates transcription initiation by recruiting components of the RNA polymerase II machinery. Studies indicate that TBPL1 is essential for embryonic development, as knockout models in mice result in early embryonic lethality due to defects in zygotic genome activation.
The recombinant TBPL1 protein is engineered for in vitro studies, typically produced using bacterial or eukaryotic expression systems. Its purification enables functional analyses, such as DNA-binding assays, protein interaction studies, and transcriptional activation experiments. Researchers utilize TBPL1 recombinant protein to explore its role in cell differentiation, tissue-specific gene regulation, and its interplay with other transcription factors or coactivators.
Dysregulation of TBPL1 has been linked to reproductive disorders and developmental abnormalities, making it a target for studying infertility and early developmental defects. Its unique properties and restricted expression profile position TBPL1 as a key player in understanding the complexity of transcriptional regulation during gametogenesis and embryogenesis.
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