| 纯度 | >80%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TBC1D22B |
| Uniprot No | Q9NU19 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-505aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMAAENSK QFWKRSAKLP GSIQPVYGAQ HPPLDPRLTK NFIKERSKVN TVPLKNKKAS SFHEFARNTS DAWDIGDDEE EDFSSPSFQT LNSKVALATA AQVLENHSKL RVKPERSQST TSDVPANYKV IKSSSDAQLS RNSSDTCLRN PLHKQQSLPL RPIIPLVARI SDQNASGAPP MTVREKTRLE KFRQLLSSQN TDLDELRKCS WPGVPREVRP ITWRLLSGYL PANTERRKLT LQRKREEYFG FIEQYYDSRN EEHHQDTYRQ IHIDIPRTNP LIPLFQQPLV QEIFERILFI WAIRHPASGY VQGINDLVTP FFVVFLSEYV EEDVENFDVT NLSQDMLRSI EADSFWCMSK LLDGIQDNYT FAQPGIQKKV KALEELVSRI DEQVHNHFRR YEVEYLQFAF RWMNNLLMRE LPLRCTIRLW DTYQSEPEGF SHFHLYVCAA FLIKWRKEIL DEEDFQGLLM LLQNLPTIHW GNEEIGLLLA EAYRLKYMFA DAPNHYRR |
| 预测分子量 | 62 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TBC1D22B重组蛋白的参考文献示例(注：以下内容为模拟生成，实际文献可能需要进一步验证)：
1. **文献名称**: *"TBC1D22B interacts with viral proteins to facilitate virion assembly"*
**作者**: Smith J, et al.
**摘要**: 本研究通过重组TBC1D22B蛋白的体外表达和免疫共沉淀实验，发现其与多种包膜病毒(如疱疹病毒)的结构蛋白相互作用，可能通过调控细胞内运输通路促进病毒粒子的组装和释放。
2. **文献名称**: *"Structural characterization of recombinant TBC1D22B and its role in Rab GTPase regulation"*
**作者**: Zhang L, et al.
**摘要**: 利用重组TBC1D22B蛋白的晶体结构解析，揭示了其TBC结构域与Rab11 GTP酶的特异性结合机制，表明该蛋白在细胞膜运输囊泡的定向移动中起关键作用。
3. **文献名称**: *"TBC1D22B recombinant protein suppresses tumor cell migration by modulating autophagy pathways"*
**作者**: Lee S, et al.
**摘要**: 通过体外细胞实验发现，外源性重组TBC1D22B蛋白可抑制癌细胞的迁移能力，机制与自噬相关蛋白LC3的磷酸化水平变化及溶酶体功能调控相关。
4. **文献名称**: *"Proteomic analysis of TBC1D22B recombinant protein interactions in neuronal cells"*
**作者**: Chen R, et al.
**摘要**: 利用重组TBC1D22B蛋白的亲和纯化-质谱联用技术，鉴定出其在神经元细胞中与突触运输相关的多个伴侣蛋白(如KIF5A、Dynamin-1)，提示其在神经突触可塑性中的潜在功能。
**注意**：以上为示例性内容，实际文献需通过PubMed、Google Scholar等平台以关键词“TBC1D22B recombinant”或“TBC1D22B function”检索。若研究较少，可扩展至相关家族蛋白(如TBC1D22A)或功能类似蛋白的文献参考。
TBC1D22B is a member of the TBC (Tre-2/Bub2/Cdc16) domain-containing protein family, which is broadly implicated in regulating intracellular vesicle trafficking and membrane dynamics. These proteins typically function as GTPase-activating proteins (GAPs) targeting Rab GTPases, key regulators of vesicle transport, fusion, and organelle organization. TBC1D22B contains a conserved TBC domain responsible for Rab inactivation by hydrolyzing GTP to GDP, though its specific Rab substrates and precise cellular roles remain under investigation.
The gene encoding TBC1D22B is located on human chromosome 15q22.31 and is expressed in multiple tissues, with higher levels observed in the brain, suggesting potential neurological functions. Studies link TBC1D22B to lysosomal positioning, autophagy, and endosomal sorting, processes critical for cellular homeostasis. Dysregulation of these pathways is associated with neurodegenerative diseases and cancer, implicating TBC1D22B in pathological contexts. For instance, altered TBC1D22B expression has been reported in glioblastoma and hepatocellular carcinoma, though mechanistic insights are limited.
Recombinant TBC1D22B protein is engineered for in vitro studies to elucidate its biochemical interactions, substrate specificity, and regulatory mechanisms. Produced via bacterial or mammalian expression systems, the purified protein enables structural analysis (e.g., crystallography), GAP activity assays, and interaction mapping with Rab proteins or binding partners. Such research aims to clarify its role in membrane trafficking networks and disease pathways. Current challenges include identifying its physiological Rab targets and validating disease-related mutations. As a relatively understudied TBC member, TBC1D22B represents a frontier in understanding vesicle trafficking regulation and its therapeutic potential in cancer or lysosomal disorders.
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