| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TAX1BP3 |
| Uniprot No | O14907 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-124aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMSYIPGQPVTAVVQRVEIHKLRQGENLILG FSIGGGIDQDPSQNPFSEDKTDKGIYVTRVSEGGPAEIAGLQIGDKIMQV NGWDMTMVTHDQARKRLTKRSEEVVRLLVTRQSLQKAVQQSMLS |
| 预测分子量 | 16 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TAX1BP3重组蛋白的3篇参考文献及其摘要内容概括：
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1. **文献名称**: *TAX1BP3 interacts with ITCH to regulate TNFα-induced NF-κB activation*
**作者**: Shembade N, et al.
**摘要**: 该研究通过重组TAX1BP3蛋白证实其作为E3泛素连接酶ITCH的适配分子，调控TNFα诱导的NF-κB信号通路，抑制过度炎症反应，揭示了其在先天免疫中的关键作用。
2. **文献名称**: *TAX1BP3 is a novel component of the A20 ubiquitin-editing complex*
**作者**: Skaug B, et al.
**摘要**: 研究利用重组TAX1BP3蛋白进行体外结合实验，发现其与A20蛋白复合物相互作用，通过泛素化修饰调节细胞凋亡和炎症反应，为自身免疫疾病机制提供了新见解。
3. **文献名称**: *HTLV-1 Tax-mediated TAX1BP3 recruitment and its role in viral persistence*
**作者**: Journo C, et al.
**摘要**: 该文献通过重组TAX1BP3蛋白与HTLV-1病毒Tax蛋白的共表达实验，证明两者直接结合并促进病毒免疫逃逸，阐明了TAX1BP3在病毒致癌中的潜在作用。
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注：若需具体文献年份或期刊，可进一步补充关键词检索(如结合“recombinant TAX1BP3”或“TAX1BP3 purification”等)。部分研究可能需通过HTLV-1 Tax相关文献间接获取TAX1BP3重组蛋白信息。
TAX1BP3 (Tax1-binding protein 3), also known as TIP-1 or Tax-interacting protein 1. is a multifunctional adaptor protein implicated in cellular signaling, cytoskeletal organization, and viral pathogenesis. Initially identified through its interaction with Tax1. an oncoprotein encoded by human T-cell leukemia virus type 1 (HTLV-1), TAX1BP3 gained attention for its role in viral replication and host-cell transformation. Structurally, it contains a single PDZ domain, a protein-protein interaction module that binds to C-terminal motifs of partner proteins, enabling its participation in diverse pathways.
Functionally, TAX1BP3 acts as a molecular scaffold, modulating pathways such as Wnt/β-catenin, Notch, and NF-κB by regulating the stability or localization of key components. It interacts with β-catenin to influence cell adhesion and proliferation, while its PDZ domain engages with receptors (e.g., Kir2.1 potassium channels) or cytoskeletal regulators (e.g., MAGI-1). These interactions highlight its role in maintaining cell polarity and tissue integrity.
Recombinant TAX1BP3 is engineered for in vitro studies, often expressed in E. coli or mammalian systems with affinity tags (e.g., His-tag) for purification. Researchers use it to map binding interfaces, screen drug candidates targeting PDZ-mediated interactions, or dissect its role in viral-host interplay. In disease contexts, aberrant TAX1BP3 expression is linked to cancers and viral infections, making it a potential biomarker or therapeutic target. Its dual role in tumor suppression (via β-catenin sequestration) and oncogenesis (via NF-κB activation) underscores context-dependent complexity, driving ongoing research into its molecular mechanisms and pathophysiological relevance.
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