| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TALDO1 |
| Uniprot No | P37837 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-337aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMSSSPVKRQRMESALDQLKQFTTVVADTGD FHAIDEYKPQDATTNPSLILAAAQMPAYQELVEEAIAYGRKLGGSQEDQI KNAIDKLFVLFGAEILKKIPGRVSTEVDARLSFDKDAMVARARRLIELYK EAGISKDRILIKLSSTWEGIQAGKELEEQHGIHCNMTLLFSFAQAVACAE AGVTLISPFVGRILDWHVANTDKKSYEPLEDPGVKSVTKIYNYYKKFSYK TIVMGASFRNTGEIKALAGCDFLTISPKLLGELLQDNAKLVPVLSAKAAQ ASDLEKIHLDEKSFRWLHNEDQMAVEKLSDGIRKFAADAVKLERMLTERM FNAENGK |
| 预测分子量 | 40 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的关于TALDO1重组蛋白的参考文献示例(非真实文献，仅供格式参考)：
---
1. **文献名称**：*Structural characterization of recombinant human TALDO1 and its role in the pentose phosphate pathway*
**作者**：Smith A, et al.
**摘要**：研究报道了在大肠杆菌中高效表达并纯化人源TALDO1重组蛋白的过程，通过X射线晶体学解析了其三维结构，揭示了底物结合位点的关键氨基酸残基，并验证了其催化转醛醇酶活性的动力学参数。
---
2. **文献名称**：*TALDO1 recombinant protein mitigates oxidative stress in cellular models of metabolic syndrome*
**作者**：Chen L, et al.
**摘要**：利用HEK293细胞表达重组TALDO1蛋白，证明其通过增强磷酸戊糖途径的代谢通量，显著提升细胞NADPH水平，缓解高糖诱导的氧化应激，为代谢综合征治疗提供潜在靶点。
---
3. **文献名称**：*Development of a high-throughput assay for TALDO1 enzymatic activity using recombinant protein*
**作者**：Kimura T, et al.
**摘要**：本研究建立了一种基于重组TALDO1蛋白的荧光标记高通量酶活检测方法，用于筛选小分子抑制剂，并成功鉴定出两种具有潜在药理活性的化合物。
---
4. **文献名称**：*Functional analysis of TALDO1 mutations in cancer using recombinant variants*
**作者**：García-Rojas M, et al.
**摘要**：通过构建携带癌症相关突变的TALDO1重组蛋白，发现某些突变导致酶活丧失，并引发细胞内核苷酸合成障碍，揭示了TALDO1在肿瘤代谢中的关键作用。
---
**注意**：以上文献为模拟生成，实际研究需通过PubMed、Web of Science等学术数据库检索关键词“TALDO1 recombinant protein”或结合具体研究领域筛选。
TALDO1 (transaldolase 1) is a key enzyme in the pentose phosphate pathway (PPP), a metabolic route critical for maintaining cellular redox balance and nucleotide biosynthesis. It catalyzes the reversible transfer of a three-carbon ketol unit between sugar phosphates, linking the glycolytic and PPP pathways. TALDO1 plays a vital role in regulating NADPH production, ribose synthesis, and redox homeostasis, making it essential for cell proliferation, antioxidant defense, and lipid metabolism.
Recombinant TALDO1 protein is engineered using molecular cloning techniques, typically expressed in bacterial (e.g., E. coli) or mammalian systems to ensure proper folding and enzymatic activity. Its production enables detailed biochemical studies, including enzyme kinetics, substrate specificity, and interaction analyses. Researchers utilize recombinant TALDO1 to investigate metabolic dysregulation in diseases such as cancer, diabetes, and rare genetic disorders like TALDO1 deficiency syndrome—a condition linked to mutations causing liver dysfunction, developmental delays, and hematologic abnormalities.
Additionally, recombinant TALDO1 serves as a tool for drug discovery, particularly in targeting metabolic vulnerabilities in cancer cells reliant on PPP upregulation. Structural studies using recombinant protein have revealed insights into its catalytic mechanism and potential allosteric regulatory sites. Its role in oxidative stress responses and immune modulation further underscores its therapeutic relevance. By providing a purified, functional form of the enzyme, recombinant TALDO1 accelerates both basic research and translational applications in metabolic disease and oncology.
×
