| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TAF9 |
| Uniprot No | Q16594 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-264aa |
| 氨基酸序列 | MESGKTASPKSMPKDAQMMAQILKDMGITEYEPRVINQMLEFAFRYVTTILDDAKIYSSHAKKATVDADDVRLAIQCRADQSFTSPPPRDFLLDIARQRNQTPLPLIKPYSGPRLPPDRYCLTAPNYRLKSLQKKASTSAGRITVPRLSVGSVTSRPSTPTLGTPTPQTMSVSTKVGTPMSLTGQRFTVQMPTSQSPAVKASIPATSAVQNVLINPSLIGSKNILITTNMMSSQNTANESSNALKRKREDDDDDDDDDDDYDNL |
| 预测分子量 | 34.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇与TAF9重组蛋白相关的研究文献示例：
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1. **文献名称**：*Structural insights into TAF9 oligomerization and its interaction with TAF6*
**作者**：Leila M. Forouzan et al.
**摘要**：本研究通过重组表达人源TAF9蛋白，结合X射线晶体学解析了其二聚体结构，揭示了TAF9与TAF6相互作用的关键位点，为TFIID复合物组装机制提供了结构基础。
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2. **文献名称**：*TAF9 regulates gene-specific autophagy by interacting with histone H3K4me3*
**作者**：Jin Zhang, Wei Xie
**摘要**：利用大肠杆菌表达的重组TAF9蛋白，作者发现其通过识别组蛋白H3K4三甲基化修饰参与自噬相关基因的转录调控，扩展了TAF9在表观遗传调控中的功能认知。
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3. **文献名称**：*Recombinant TAF9 as a tool for studying RNA polymerase II transcription initiation*
**作者**：Maria S. Rodriguez et al.
**摘要**：该研究优化了TAF9在昆虫细胞中的重组表达系统，纯化后的蛋白成功重建了体外转录体系，证明TAF9在RNA聚合酶II介导的启动子识别中具有关键作用。
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**备注**：以上文献信息为模拟示例，实际文献需通过PubMed、Web of Science等数据库检索。如需具体文献，建议补充研究背景(如物种、功能方向)以便精准推荐。
TAF9 (TATA-box binding protein-associated factor 9) is a conserved subunit of the TFIID complex, a critical general transcription factor required for RNA polymerase II-mediated gene expression. As part of the TFIID complex, TAF9 plays a role in promoter recognition, nucleating the assembly of the pre-initiation complex. It interacts with TBP (TATA-binding protein) and other TAFs to stabilize DNA binding and regulate transcription initiation. Structurally, TAF9 contains a histone-fold domain (HFD) that mediates dimerization with TAF6. forming a heterodimer essential for TFIID integrity. Beyond transcription initiation, TAF9 participates in chromatin modification by associating with histone acetyltransferase complexes like PCAF/GCN5.
Recombinant TAF9 protein is produced using heterologous expression systems (e.g., E. coli, mammalian cells) to study its molecular interactions and structural biology. Its recombinant form enables biochemical assays such as protein-protein interaction mapping, crystallography, and in vitro transcription analysis. Researchers utilize it to dissect TFIID assembly mechanisms, investigate transcriptional dysregulation in diseases (e.g., cancers, neurological disorders), and explore its role in cell cycle regulation. Recombinant TAF9 also serves as an antigen for antibody production. Compared to native purification, recombinant technology ensures higher purity, avoids contamination from endogenous TFIID components, and allows site-specific mutagenesis for functional studies. Recent studies highlight its involvement in stress-responsive gene networks and viral transcription hijacking, underscoring its broad biological relevance.
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