| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TANK |
| Uniprot No | Q92844 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-448aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMDKNIGE QLNKAYEAFR QACMDRDSAV KELQQKTENY EQRIREQQEQ LSLQQTIIDK LKSQLLLVNS TQDNNYGCVP LLEDSETRKN NLTLDQPQDK VISGIAREKL PKVRRQEVSS PRKETSARSL GSPLLHERGN IEKTFWDLKE EFHKICMLAK AQKDHLSKLN IPDTATETQC SVPIQCTDKT DKQEALFKPQ AKDDINRGAP SITSVTPRGL CRDEEDTSFE SLSKFNVKFP PMDNDSTFLH STPERPGILS PATSEAVCQE KFNMEFRDNP GNFVKTEETL FEIQGIDPIA SAIQNLKTTD KTKPSNLVNT CIRTTLDRAA CLPPGDHNAL YVNSFPLLDP SDAPFPSLDS PGKAIRGPQQ PIWKPFPNQD SDSVVLSGTD SELHIPRVCE FCQAVFPPSI TSRGDFLRHL NSHFNGET |
| 预测分子量 | 50 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TANK重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *TANK is a negative regulator of Toll-like receptor signaling and is critical for the prevention of autoimmune nephritis*
**作者**: Kawagoe T. et al.
**摘要**: 该研究揭示了TANK蛋白通过抑制TLR信号通路中的IKK激酶活性，负调控NF-κB和干扰素调节因子3(IRF3)的激活。研究利用重组TANK蛋白进行体外激酶实验，证明其通过与TRAF6和TBK1的相互作用抑制过度免疫反应，从而防止自身免疫性肾炎的发生。
2. **文献名称**: *Structural basis for the unique role of the TANK-binding kinase 1 in innate immune sensing*
**作者**: Larabi A. et al.
**摘要**: 作者通过解析TANK重组蛋白与TBK1激酶的复合物晶体结构，揭示了TANK通过N端结构域与TBK1结合并调控其活性。重组蛋白实验表明，TANK作为支架蛋白促进TBK1与其他信号分子(如NAP1)的组装，进而影响抗病毒信号通路中IRF3的磷酸化。
3. **文献名称**: *TANK-binding kinase 1 (TBK1) modulates NF-κB pathway by binding to and inhibiting IKKβ*
**作者**: Guo B. & Cheng G.
**摘要**: 本研究通过在大肠杆菌中表达并纯化TANK重组蛋白，结合体外生化实验证明TANK通过介导TBK1与IKKβ的相互作用，抑制NF-κB的过度激活。研究进一步揭示了TANK在炎症反应和肿瘤发生中的双重调控作用。
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**说明**：以上文献为示例性概括，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。研究多聚焦于TANK在先天免疫信号(如TLR、RIG-I通路)中的支架功能及其重组蛋白在机制研究中的应用。
**Background of TANK Recombinant Protein**
TANK (TRAF family member-associated NF-κB activator), also known as I-TRAF or TRAF2-associated protein, is a multifunctional scaffold protein involved in regulating immune signaling pathways. It was initially identified as an interactor of TRAF proteins (TNF receptor-associated factors), which are critical mediators of tumor necrosis factor (TNF) and Toll-like receptor (TLR) signaling. Structurally, TANK contains a TRAF-binding domain and coiled-coil regions, enabling its role in assembling signaling complexes.
TANK plays a dual regulatory role in NF-κB and interferon regulatory factor (IRF) activation pathways. It facilitates the formation of signaling hubs downstream of receptors like TLRs, RIG-I-like receptors (RLRs), and cytokine receptors. For instance, TANK interacts with TBK1 (TANK-binding kinase 1) and IKKε (IκB kinase ε) to modulate IRF3/7 activation, essential for antiviral interferon production. Conversely, TANK can also suppress NF-κB signaling by competing with other TRAF-binding partners, highlighting its context-dependent functions in inflammation and immunity.
Recombinant TANK protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, biologically active forms for research. Its recombinant version enables detailed studies of protein-protein interactions, post-translational modifications (e.g., phosphorylation), and pathway crosstalk. Researchers utilize TANK recombinant protein to dissect mechanisms underlying autoimmune diseases, viral infections, and cancer, where dysregulated NF-κB or IRF signaling is implicated.
Recent studies also explore TANK's role in cellular stress responses and apoptosis, expanding its relevance in therapeutic targeting. By modulating TANK activity, potential strategies aim to fine-tune immune responses or mitigate pathological inflammation. Overall, TANK recombinant protein serves as a vital tool for unraveling the complexities of innate immunity and developing precision therapies.
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