| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TATDN3 |
| Uniprot No | Q17R31 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-274aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMRAAGV GLVDCHCHLS APDFDRDLDD VLEKAKKANV VALVAVAEHS GEFEKIMQLS ERYNGFVLPC LGVHPVQGLP PEDQRSVTLK DLDVALPIIE NYKDRLLAIG EVGLDFSPRF AGTGEQKEEQ RQVLIRQIQL AKRLNLPVNV HSRSAGRPTI NLLQEQGAEK VLLHAFDGRP SVAMEGVRAG YFFSIPPSII RSGQKQKLVK QLPLTSICLE TDSPALGPEK QVRNEPWNIS ISAEYIAQVK GISVEEVIEV TTQNALKLFP KLRHLLQK |
| 预测分子量 | 33 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为模拟生成的参考文献示例(请注意这些文献信息为虚构，建议通过学术数据库查询真实研究)：
1. **标题**: "Structural and Functional Characterization of Recombinant TATDN3 Protein"
**作者**: Zhang L, et al.
**摘要**: 本研究通过原核表达系统成功纯化了TATDN3重组蛋白，解析了其晶体结构，证实其具有Mg²⁺依赖的核酸酶活性，并揭示了其在DNA损伤修复中的潜在作用。
2. **标题**: "TATDN3 Modulates Apoptosis via Mitochondrial DNA Degradation"
**作者**: Chen X, et al.
**摘要**: 文章发现TATDN3重组蛋白可通过切割线粒体DNA触发细胞凋亡通路，为癌症治疗中靶向线粒体功能的药物开发提供了新思路。
3. **标题**: "TATDN3 Recombinant Protein as a Biomarker in Colorectal Cancer"
**作者**: Wang Y, et al.
**摘要**: 通过临床样本分析，发现TATDN3在结直肠癌组织中高表达，重组蛋白实验证明其通过调控肿瘤细胞基因组不稳定性促进疾病进展。
4. **标题**: "Optimizing Recombinant TATDN3 Expression in Mammalian Systems"
**作者**: Gupta R, et al.
**摘要**: 本研究改进了哺乳动物细胞中TATDN3重组蛋白的表达工艺，提高了蛋白产量及稳定性，为后续功能研究及药物筛选提供了可靠工具。
**建议**：实际研究中请通过PubMed、Web of Science等平台，以关键词“TATDN3 recombinant protein”或“TATDN3 function”检索最新文献。
**Background of TATDN3 Recombinant Protein**
TATDN3 (TatD-like DNase III) belongs to the TatD family of nucleases, initially identified in bacterial systems like *Escherichia coli* as part of the twin-arginine translocation (Tat) pathway. While bacterial TatD proteins are associated with DNA repair and degradation, eukaryotic homologs, including TATDN3. exhibit conserved nuclease activity but diverge in biological roles. TATDN3 is classified as a magnesium-dependent DNase, structurally characterized by a conserved metalloenzyme fold critical for hydrolyzing DNA substrates.
In humans, TATDN3 is implicated in maintaining genomic stability, particularly in resolving replication stress and DNA damage responses. Studies suggest its involvement in apoptotic DNA fragmentation and mitochondrial DNA metabolism, linking it to cellular homeostasis and disease pathways. Dysregulation of TATDN3 has been observed in certain cancers, where aberrant DNA repair mechanisms contribute to tumor progression or chemoresistance.
Recombinant TATDN3 is typically produced via heterologous expression systems (e.g., *E. coli* or mammalian cells*) to study its biochemical properties and interactions. The protein is purified using affinity tags (e.g., His-tag) and chromatography, enabling functional assays to dissect its enzymatic kinetics, substrate specificity, and regulatory partners. Its recombinant form has also been leveraged to explore therapeutic targeting, such as modulating nuclease activity in cancer or inflammatory conditions.
Current research focuses on elucidating TATDN3’s role in chromatin remodeling, crossover formation during meiosis, and crosstalk with DNA repair machinery like homologous recombination. Evolutionary conservation across prokaryotes and eukaryotes underscores its fundamental importance in nucleic acid metabolism, making it a compelling subject for both basic and translational studies in genomics and molecular medicine.
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