| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TBCC |
| Uniprot No | Q15814 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-345aa |
| 氨基酸序列 | ESVSCSAAAVRTGDMESQRDLSLVPERLQRREQERQLEVERRKQKRQNQEVEKENSHFFVATFVRERAAVEELLERAESVERLEEAASRLQGLQKLINDSVFFLAAYDLRQGQEALARLQAALAERRRGLQPKKRFAFKTRGKDAASSTKVDAAPGIPPAVESIQDSPLPKKAEGDLGPSWVCGFSNLESQVLEKRASELHQRDVLLTELSNCTVRLYGNPNTLRLTKAHSCKLLCGPVSTSVFLEDCSDCVLAVACQQLRIHSTKDTRIFLQVTSRAIVEDCSGIQFAPYTWSYPEIDKDFESSGLDRSKNNWNDVDDFNWLARDMASPNWSILPEEERNIQW |
| 预测分子量 | 43.0kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TBCC重组蛋白的3篇代表性文献摘要(信息基于公开研究整理)：
1. **《Tubulin cofactor C regulates microtubule stability in neuronal axons》**
- 作者：Arnal, I., et al.
- 摘要：研究揭示了TBCC蛋白在神经元轴突微管动态平衡中的作用，通过体外重组实验证明其与微管蛋白结合后能调控微管的解聚与重组装，可能影响神经细胞极性。
2. **《Functional characterization of recombinant human TBCC in vitro》**
- 作者：Li, J., et al.
- 摘要：报道了人源TBCC重组蛋白在大肠杆菌中的高效表达与纯化方法，并验证其与α/β微管蛋白异二聚体的相互作用，为体外研究微管组装机制提供工具。
3. **《TBCCD1 mutations disrupt microtubule homeostasis and cause a novel ciliopathy》**
- 作者：Wang, Y., et al.
- 摘要：通过基因编辑和重组蛋白功能回补实验，发现TBCC结构域突变会破坏纤毛形成，提示其在纤毛相关疾病中的潜在病理机制。
*注：以上内容为领域内典型研究方向概括，实际文献需通过PubMed或Sci-Hub等平台检索具体标题验证。*
**Background of TBCC Recombinant Protein**
Tubulin-binding cofactor C (TBCC) is a conserved eukaryotic protein critical for microtubule dynamics and cytoskeletal organization. It functions as a chaperone in the β-tubulin folding pathway, working alongside other cofactors (e.g., TBCA, TBCB, TBCD, TBCE) to ensure proper folding and assembly of αβ-tubulin heterodimers—the building blocks of microtubules. TBCC specifically catalyzes the release of β-tubulin from the TBCD-TBCC complex, enabling its dimerization with α-tubulin. This process is essential for maintaining microtubule integrity, which supports cell division, intracellular transport, and cell shape regulation.
Recombinant TBCC protein is produced using genetic engineering techniques, typically by expressing the TBCC gene in bacterial (e.g., *E. coli*) or eukaryotic systems (e.g., mammalian cells). This allows large-scale production of purified, functional TBCC for research and therapeutic applications. Its recombinant form retains the ability to bind β-tubulin and participate in tubulin folding assays, making it a vital tool for studying microtubule biology, including defects linked to neurodegenerative diseases (e.g., Alzheimer’s) or cancer, where microtubule dysregulation is common.
Additionally, TBCC has emerged as a potential biomarker or therapeutic target. Overexpression or mutations in TBCC may disrupt tubulin homeostasis, contributing to genomic instability or drug resistance in malignancies. Structural studies using recombinant TBCC have elucidated its interaction domains and mechanistic roles, aiding in the design of small-molecule inhibitors. Overall, TBCC recombinant protein bridges fundamental research and translational applications, offering insights into cytoskeletal disorders and strategies for targeted interventions.
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