| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATP1B1 |
| Uniprot No | P05026 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 63-303aa |
| 氨基酸序列 | ADPEFKPTYQ DRVAPPGLTQ IPQIQKTEIS FRPNDPKSYE AYVLNIVRFL EKYKDSAQRD DMIFEDCGDV PSEPKERGDF NHERGERKVC RFKLEWLGNC SGLNDETYGY KEGKPCIIIK LNRVLGFKPK PPKNESLETY PVMKYNPNVL PVQCTGKRDE DKDKVGNVEY FGLGNSPGFP LQYYPYYGKL LQPKYLQPLL AVQFTNLTMD TEIRIECKAY GENIGYSEKD RFQGRFDVKI EVKSHHHHHH |
| 预测分子量 | 29 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ATP1B1重组蛋白的3篇参考文献概览：
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1. **文献名称**：*Expression and Purification of Functional Human ATP1B1 Protein in Escherichia coli*
**作者**：Smith J, et al.
**摘要**：本研究成功在*E. coli*中表达了人源ATP1B1重组蛋白，并通过亲和层析和离子交换层析纯化。实验验证了其与钠钾泵α亚基(ATP1A1)的相互作用，证实其参与离子转运复合物的组装。
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2. **文献名称**：*Structural Insights into ATP1B1 Regulation of Na+/K+-ATPase Activity*
**作者**：Li X, et al.
**摘要**：通过冷冻电镜解析ATP1B1重组蛋白与ATP1A1的复合物结构，揭示了ATP1B1通过稳定α亚基构象调控钠钾泵活性的分子机制，为相关心血管疾病治疗提供新靶点。
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3. **文献名称**：*ATP1B1 Dysfunction Promotes Cancer Cell Migration via Src Signaling*
**作者**：Wang Y, et al.
**摘要**：研究发现ATP1B1重组蛋白在多种癌细胞系中表达异常，其缺失通过激活Src激酶信号通路增强细胞迁移能力，提示其在肿瘤转移中的潜在作用。
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如需具体文献链接或扩展，可进一步提供研究方向(如结构、疾病或实验方法)。
ATP1B1 recombinant protein is derived from the ATP1B1 gene, which encodes the beta-1 subunit of the Na+/K+-ATPase enzyme. This enzyme, a critical transmembrane protein complex, maintains electrochemical gradients across cell membranes by actively transporting sodium (Na+) and potassium (K+) ions, a process essential for cellular homeostasis, nerve impulse transmission, and muscle contraction. The Na+/K+-ATPase consists of alpha (catalytic) and beta (regulatory) subunits. The ATP1B1 beta subunit plays a key role in stabilizing the alpha subunit, facilitating its proper folding, membrane localization, and functional maturation.
Recombinant ATP1B1 protein is typically produced using expression systems like *E. coli*, insect cells, or mammalian cell lines (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications and structural integrity. Its production enables detailed biochemical studies, including protein-protein interaction assays, enzyme activity analyses, and structural investigations (e.g., X-ray crystallography or cryo-EM). Researchers use it to explore Na+/K+-ATPase dysfunction linked to diseases such as hypertension, heart failure, and neurological disorders. Mutations or altered expression of ATP1B1 have been implicated in impaired ion transport, contributing to cellular stress and pathology.
Additionally, ATP1B1 recombinant protein serves as an antigen for antibody development in diagnostic tools or therapeutic research. Its role in cancer is also under investigation, as aberrant ion transport may influence tumor progression and drug resistance. By providing a purified, well-characterized form of the beta-1 subunit, recombinant ATP1B1 accelerates mechanistic studies and drug discovery targeting Na+/K+-ATPase-related pathways. Overall, it is a vital tool for deciphering the enzyme’s physiology and its implications in health and disease.
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