首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠髓样分化因子88(MyD88)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的MYD88含量。MYD88 是一种关键接头蛋白，在先天免疫信号通路中发挥重要作用。其突变与多种血液系统恶性肿瘤相关，如 Waldenström 巨球蛋白血症。研究机制聚焦于它参与 Toll 样受体和白细胞介素-1 受体信号转导，促使下游信号级联反应，为相关疾病治疗提供潜在靶点。试剂盒检测范围为47 pg/mL-3000 pg/mL，特别适用于免疫信号通路机制研究、炎症相关疾病模型评估以及药物干预实验中MyD88表达动态的追踪，为探索免疫调控机制提供可靠工具。其广泛兼容性满足体外细胞模型、动物组织样本及体液样本的检测需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Myd88 ELISA Kit; Myeloid differentiation primary response protein MyD88 ELISA Kit
缩写：MYD88
Uniprot No.：P22366
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：47 pg/mL-3000 pg/mL
灵敏度：11.7 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MyD88 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 81-87 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 95-102 | |||||
| 1:4 | Average % | 110 | ||||
| Range % | 105-115 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 85-93 | |||||
回收率：
| The recovery of mouse MyD88 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 102 | 98-106 | ||||
| EDTA plasma (n=4) | 83 | 80-86 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected30002.3732.3982.3862.24415001.6761.6081.6421.5007501.0611.0101.0360.8943750.6760.6980.6870.545187.50.4460.4650.4560.314940.3530.3680.3610.219470.2390.2450.2420.10000.1410.1430.142 | ||||||
货期：3-5 working days
×
