首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠N-乙酰-β-D-氨基葡萄糖苷酶(NAG)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、尿液样本中的NAG含量。大鼠N-乙酰-β-D-氨基葡萄糖苷酶（NAG）是一种溶酶体水解酶，主要存在于近端肾小管中，与黏多糖类及糖蛋白代谢相关。其活性变化与肾小管损伤密切相关，可作为肾损伤的早期标志物。目前，NAG的研究主要集中在其生物学机制、靶点以及与肾小管疾病的关系上。试剂盒检测范围为1.56 mIU/mL-100 mIU/mL，该产品可广泛应用于肾脏疾病机制研究、药物肾毒性评估、代谢综合征模型构建等科研领域，尤其适用于动物实验中对尿液或血液样本的NAG动态监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Hexb ELISA Kit; Beta-hexosaminidase subunit beta ELISA Kit; EC 3.2.1.52 ELISA Kit; Beta-N-acetylhexosaminidase subunit beta ELISA Kit; Hexosaminidase subunit B ELISA Kit; N-acetyl-beta-glucosaminidase subunit beta ELISA Kit
缩写：NAG
Uniprot No.：Q6AXR4
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, urine
检测范围：1.56 mIU/mL-100 mIU/mL
灵敏度：0.39 mIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat NAG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-102 | |||||
| 1:4 | Average % | 87 | ||||
| Range % | 84-95 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 92-101 | |||||
回收率：
| The recovery of rat NAG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 95-105 | ||||
| EDTA plasma (n=4) | 96 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mIU/mlOD1OD2AverageCorrected1002.4952.4712.4832.306501.9551.9871.9711.794251.3561.3681.3621.18512.50.9340.9630.9490.7726.250.5870.5970.5920.4153.120.4250.4510.4380.2611.560.3610.3740.3680.19100.1750.1780.177 | ||||||
货期：3-5 working days
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